Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon

Tang Yi Tsao; Chin Shaw Stella Tsai; Jai Nien Tung; Shun Liang Chen; Chia Herng Yue; Ching Fong Liao; Chi Chao Wang; Ming Chung Jiang

doi:10.1007/s11010-009-0054-0

Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon

Tang Yi Tsao, Chin Shaw Stella Tsai, Jai Nien Tung, Shun Liang Chen, Chia Herng Yue, Ching Fong Liao, Chi Chao Wang, Ming Chung Jiang

School of Medicine

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The secretion of colorectal epithelium is important for maintaining the physiological function of colorectal organ. Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. Polarity is essential for directed secretion of substances produced by epithelial cells to the external (luminal) compartment; CAS overexpression induced polarization of HT-29 cells. CAS was punctate stained in the cytoplasm of HT-29 cells, and CAS overexpression increased the translocation of CAS-stained vesicles to the cytoplasm near cell membrane and cell protrusions. CAS overexpression increased the secretion of carcinoembryonic antigen (CEA) and cathepsin D. Immunohistochemistry showed CAS was positively stained in the goblet cells of colon mucosa and cells in the crypts of Lieberkühn of human colon as well as the glands in metastatic colorectal cancer tissue. Our results suggest that CAS regulates the secretion of colorectal cells and may regulate the metastasis of colorectal cancer.

Original language	English
Pages (from-to)	163-170
Number of pages	8
Journal	Molecular and Cellular Biochemistry
Volume	327
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-009-0054-0
Publication status	Published - 2009

Keywords

CAS
Colon
Colorectal cancer
Polarity
Secretion

ASJC Scopus subject areas

Clinical Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11010-009-0054-0

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title = "Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon",

abstract = "The secretion of colorectal epithelium is important for maintaining the physiological function of colorectal organ. Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. Polarity is essential for directed secretion of substances produced by epithelial cells to the external (luminal) compartment; CAS overexpression induced polarization of HT-29 cells. CAS was punctate stained in the cytoplasm of HT-29 cells, and CAS overexpression increased the translocation of CAS-stained vesicles to the cytoplasm near cell membrane and cell protrusions. CAS overexpression increased the secretion of carcinoembryonic antigen (CEA) and cathepsin D. Immunohistochemistry showed CAS was positively stained in the goblet cells of colon mucosa and cells in the crypts of Lieberk{\"u}hn of human colon as well as the glands in metastatic colorectal cancer tissue. Our results suggest that CAS regulates the secretion of colorectal cells and may regulate the metastasis of colorectal cancer.",

keywords = "CAS, Colon, Colorectal cancer, Polarity, Secretion",

author = "Tsao, {Tang Yi} and Tsai, {Chin Shaw Stella} and Tung, {Jai Nien} and Chen, {Shun Liang} and Yue, {Chia Herng} and Liao, {Ching Fong} and Wang, {Chi Chao} and Jiang, {Ming Chung}",

year = "2009",

doi = "10.1007/s11010-009-0054-0",

language = "English",

volume = "327",

pages = "163--170",

journal = "Molecular and Cellular Biochemistry",

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AU - Tsao, Tang Yi

AU - Tsai, Chin Shaw Stella

AU - Tung, Jai Nien

AU - Chen, Shun Liang

AU - Yue, Chia Herng

AU - Liao, Ching Fong

AU - Wang, Chi Chao

AU - Jiang, Ming Chung

PY - 2009

Y1 - 2009

N2 - The secretion of colorectal epithelium is important for maintaining the physiological function of colorectal organ. Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. Polarity is essential for directed secretion of substances produced by epithelial cells to the external (luminal) compartment; CAS overexpression induced polarization of HT-29 cells. CAS was punctate stained in the cytoplasm of HT-29 cells, and CAS overexpression increased the translocation of CAS-stained vesicles to the cytoplasm near cell membrane and cell protrusions. CAS overexpression increased the secretion of carcinoembryonic antigen (CEA) and cathepsin D. Immunohistochemistry showed CAS was positively stained in the goblet cells of colon mucosa and cells in the crypts of Lieberkühn of human colon as well as the glands in metastatic colorectal cancer tissue. Our results suggest that CAS regulates the secretion of colorectal cells and may regulate the metastasis of colorectal cancer.

AB - The secretion of colorectal epithelium is important for maintaining the physiological function of colorectal organ. Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. Polarity is essential for directed secretion of substances produced by epithelial cells to the external (luminal) compartment; CAS overexpression induced polarization of HT-29 cells. CAS was punctate stained in the cytoplasm of HT-29 cells, and CAS overexpression increased the translocation of CAS-stained vesicles to the cytoplasm near cell membrane and cell protrusions. CAS overexpression increased the secretion of carcinoembryonic antigen (CEA) and cathepsin D. Immunohistochemistry showed CAS was positively stained in the goblet cells of colon mucosa and cells in the crypts of Lieberkühn of human colon as well as the glands in metastatic colorectal cancer tissue. Our results suggest that CAS regulates the secretion of colorectal cells and may regulate the metastasis of colorectal cancer.

KW - CAS

KW - Colon

KW - Colorectal cancer

KW - Polarity

KW - Secretion

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SP - 163

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JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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