TY - JOUR
T1 - Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging
AU - Huang, Huey Chung
AU - Chang, Po Yuan
AU - Chang, Karen
AU - Chen, Chao Yu
AU - Lin, Chung Wu
AU - Chen, Jyh Horng
AU - Mou, Chung Yuan
AU - Chang, Zee Fen
AU - Chang, Fu Hsiung
N1 - Funding Information:
This study was supported by grants NSC 94-2311-B-002-029 and NSC 95-2311-B-002-023 from the National Science Council, and NTUH 95S342 from the National Taiwan University Hospital, Taipei, Taiwan. We thank Dr. Yann Hung for her helpful suggestions and comments on this manuscript.
PY - 2009
Y1 - 2009
N2 - Background. Application of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent has improved the quality of magnetic resonance (MR) imaging. Low efficiency of loading the commercially available iron oxide nanoparticles into cells and the cytotoxicity of previously formulated complexes limit their usage as the image probe. Here, we formulated new cationic lipid nanoparticles containing SPIOs feasible for in vivo imaging. Methods. Hydrophobic SPIOs were incorporated into cationic lipid 1,2-dioleoyl-3- (trimethylammonium) propane (DOTAP) and polyethylene-glycol-2000-1,2-distearyl- 3-sn-phosphatidylethanolamine (PEG-DSPE) based micelles by self-assembly procedure to form lipid-coated SPIOs (L-SPIOs). Trace amount of Rhodamine-dioleoyl-phosphatidylethanolamine (Rhodamine-DOPE) was added as a fluorescent indicator. Particle size and zeta potential of L-SPIOs were determined by Dynamic Light Scattering (DLS) and Laser Doppler Velocimetry (LDV), respectively. HeLa, PC-3 and Neuro-2a cells were tested for loading efficiency and cytotoxicity of L-SPIOs using fluorescent microscopy, Prussian blue staining and flow cytometry. L-SPIO-loaded CT-26 cells were tested for in vivo MR imaging. Results. The novel formulation generates L-SPIOs particle with the average size of 46 nm. We showed efficient cellular uptake of these L-SPIOs with cationic surface charge into HeLa, PC-3 and Neuro-2a cells. The L-SPIO-loaded cells exhibited similar growth potential as compared to unloaded cells, and could be sorted by a magnet stand over ten-day duration. Furthermore, when SPIO-loaded CT-26 tumor cells were injected into Balb/c mice, the growth status of these tumor cells could be monitored using optical and MR images. Conclusion. We have developed a novel cationic lipid-based nanoparticle of SPIOs with high loading efficiency, low cytotoxicity and long-term imaging signals. The results suggested these newly formulated non-toxic lipid-coated magnetic nanoparticles as a versatile image probe for cell tracking.
AB - Background. Application of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent has improved the quality of magnetic resonance (MR) imaging. Low efficiency of loading the commercially available iron oxide nanoparticles into cells and the cytotoxicity of previously formulated complexes limit their usage as the image probe. Here, we formulated new cationic lipid nanoparticles containing SPIOs feasible for in vivo imaging. Methods. Hydrophobic SPIOs were incorporated into cationic lipid 1,2-dioleoyl-3- (trimethylammonium) propane (DOTAP) and polyethylene-glycol-2000-1,2-distearyl- 3-sn-phosphatidylethanolamine (PEG-DSPE) based micelles by self-assembly procedure to form lipid-coated SPIOs (L-SPIOs). Trace amount of Rhodamine-dioleoyl-phosphatidylethanolamine (Rhodamine-DOPE) was added as a fluorescent indicator. Particle size and zeta potential of L-SPIOs were determined by Dynamic Light Scattering (DLS) and Laser Doppler Velocimetry (LDV), respectively. HeLa, PC-3 and Neuro-2a cells were tested for loading efficiency and cytotoxicity of L-SPIOs using fluorescent microscopy, Prussian blue staining and flow cytometry. L-SPIO-loaded CT-26 cells were tested for in vivo MR imaging. Results. The novel formulation generates L-SPIOs particle with the average size of 46 nm. We showed efficient cellular uptake of these L-SPIOs with cationic surface charge into HeLa, PC-3 and Neuro-2a cells. The L-SPIO-loaded cells exhibited similar growth potential as compared to unloaded cells, and could be sorted by a magnet stand over ten-day duration. Furthermore, when SPIO-loaded CT-26 tumor cells were injected into Balb/c mice, the growth status of these tumor cells could be monitored using optical and MR images. Conclusion. We have developed a novel cationic lipid-based nanoparticle of SPIOs with high loading efficiency, low cytotoxicity and long-term imaging signals. The results suggested these newly formulated non-toxic lipid-coated magnetic nanoparticles as a versatile image probe for cell tracking.
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U2 - 10.1186/1423-0127-16-86
DO - 10.1186/1423-0127-16-86
M3 - Article
C2 - 19772552
AN - SCOPUS:70350624421
SN - 1021-7770
VL - 16
JO - Journal of Biomedical Science
JF - Journal of Biomedical Science
IS - 1
M1 - 86
ER -
