TY - JOUR
T1 - Far-infrared promotes burn wound healing by suppressing NLRP3 inflammasome caused by enhanced autophagy
AU - Chiu, Hui Wen
AU - Chen, Cheng Hsien
AU - Chang, Jen Ning
AU - Chen, Chien Hsiung
AU - Hsu, Yung Ho
N1 - Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Abstract: Understanding the underlying molecular mechanisms in burn wound progression is crucial to providing appropriate diagnoses and designing therapeutic regimens for burn patients. When inflammation becomes unregulated, recurrent, or excessive, it interferes with burn wound healing. Autophagy, which is a homeostatic and catabolic degradation process, was found to protect against ischemic injury, inflammatory diseases, and apoptosis in some cases. In the present study, we investigated whether far-infrared (FIR) could ameliorate burn wound progression and promote wound healing both in vitro and in a rat model of deep second-degree burn. We found that FIR induced autophagy in differentiated THP-1 cells (human monocytic cells differentiated to macrophages). Furthermore, FIR inhibited both the NLRP3 inflammasome and the production of IL-1β in lipopolysaccharide-activated THP-1 macrophages. In addition, FIR induced the ubiquitination of ASC, which is the adaptor protein of the inflammasome, by increasing tumor necrosis factor receptor-associated factor 6 (TRAF6), which is a ubiquitin E3 ligase. Furthermore, the exposure to FIR then promoted the delivery of inflammasome to autophagosomes for degradation. In a rat burn model, FIR ameliorated burn-induced epidermal thickening, inflammatory cell infiltration, and loss of distinct collagen fibers. Moreover, FIR enhanced autophagy and suppressed the activity of the NLRP3 inflammasome in the rat skin tissue of the burn model. Based on these results, we suggest that FIR-regulated autophagy and inflammasomes will be important for the discovery of novel therapeutics to promote the healing of burn wounds. Key messages: Far-infrared (FIR) induced autophagy in THP-1 macrophages.FIR suppressed the NLRP3 inflammasome through the activation of autophagy.FIR induced the ubiquitination of ASC by increasing TRAF6.FIR ameliorated burn wound progression and promoted wound healing in a rat burn model.
AB - Abstract: Understanding the underlying molecular mechanisms in burn wound progression is crucial to providing appropriate diagnoses and designing therapeutic regimens for burn patients. When inflammation becomes unregulated, recurrent, or excessive, it interferes with burn wound healing. Autophagy, which is a homeostatic and catabolic degradation process, was found to protect against ischemic injury, inflammatory diseases, and apoptosis in some cases. In the present study, we investigated whether far-infrared (FIR) could ameliorate burn wound progression and promote wound healing both in vitro and in a rat model of deep second-degree burn. We found that FIR induced autophagy in differentiated THP-1 cells (human monocytic cells differentiated to macrophages). Furthermore, FIR inhibited both the NLRP3 inflammasome and the production of IL-1β in lipopolysaccharide-activated THP-1 macrophages. In addition, FIR induced the ubiquitination of ASC, which is the adaptor protein of the inflammasome, by increasing tumor necrosis factor receptor-associated factor 6 (TRAF6), which is a ubiquitin E3 ligase. Furthermore, the exposure to FIR then promoted the delivery of inflammasome to autophagosomes for degradation. In a rat burn model, FIR ameliorated burn-induced epidermal thickening, inflammatory cell infiltration, and loss of distinct collagen fibers. Moreover, FIR enhanced autophagy and suppressed the activity of the NLRP3 inflammasome in the rat skin tissue of the burn model. Based on these results, we suggest that FIR-regulated autophagy and inflammasomes will be important for the discovery of novel therapeutics to promote the healing of burn wounds. Key messages: Far-infrared (FIR) induced autophagy in THP-1 macrophages.FIR suppressed the NLRP3 inflammasome through the activation of autophagy.FIR induced the ubiquitination of ASC by increasing TRAF6.FIR ameliorated burn wound progression and promoted wound healing in a rat burn model.
KW - Autophagy
KW - Burn wound healing
KW - Far-infrared
KW - NLRP3 inflammasome
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U2 - 10.1007/s00109-016-1389-0
DO - 10.1007/s00109-016-1389-0
M3 - Article
C2 - 26864306
AN - SCOPUS:84957634376
SN - 0946-2716
VL - 94
SP - 809
EP - 819
JO - Journal of Molecular Medicine
JF - Journal of Molecular Medicine
IS - 7
ER -
