Facile method for determination of deoxycytidine kinase activity in biological milieus

Wei Hua Hao; Li Chieh Yang; Jong Jing Wang; Chang Shan Hsu; Li Chien Chang; Kuang Yang Hsu

doi:10.1016/j.jfda.2013.09.008

Facile method for determination of deoxycytidine kinase activity in biological milieus

Wei Hua Hao, Li Chieh Yang, Jong Jing Wang, Chang Shan Hsu, Li Chien Chang, Kuang Yang Hsu

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

Original language	English
Pages (from-to)	236-241
Number of pages	6
Journal	Journal of Food and Drug Analysis
Volume	22
Issue number	2
DOIs	https://doi.org/10.1016/j.jfda.2013.09.008
Publication status	Published - Jun 1 2014

Keywords

Chemiluminescent assay
Deoxycytidine kinase
Gemcitabine

ASJC Scopus subject areas

Food Science
Pharmacology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jfda.2013.09.008

Cite this

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title = "Facile method for determination of deoxycytidine kinase activity in biological milieus",

abstract = "A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.",

keywords = "Chemiluminescent assay, Deoxycytidine kinase, Gemcitabine",

author = "Hao, {Wei Hua} and Yang, {Li Chieh} and Wang, {Jong Jing} and Hsu, {Chang Shan} and Chang, {Li Chien} and Hsu, {Kuang Yang}",

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doi = "10.1016/j.jfda.2013.09.008",

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journal = "Journal of Food and Drug Analysis",

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T1 - Facile method for determination of deoxycytidine kinase activity in biological milieus

AU - Hao, Wei Hua

AU - Yang, Li Chieh

AU - Wang, Jong Jing

AU - Hsu, Chang Shan

AU - Chang, Li Chien

AU - Hsu, Kuang Yang

PY - 2014/6/1

Y1 - 2014/6/1

N2 - A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

AB - A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

KW - Chemiluminescent assay

KW - Deoxycytidine kinase

KW - Gemcitabine

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Facile method for determination of deoxycytidine kinase activity in biological milieus

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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