Fabrication of large perfusable macroporous cell-laden hydrogel scaffolds using microbial transglutaminase

Pei Yu Chen, Kai Chiang Yang, Chang Chin Wu, Jeen Huei Yu, Feng Huei Lin, Jui Sheng Sun

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


In this study, we developed a method to fabricate large, perfusable, macroporous, cell-laden hydrogels. This method is suitable for efficient cell seeding, and can maintain sufficient oxygen delivery and mass transfer. We first loaded three types of testing cells (including NIH 3T3, ADSC and Huh7) into gelatin hydrogel filaments, then cross-linked the cell-laden gelatin hydrogel filaments using microbial transglutaminase (mTGase). In situ cross-linking by mTGase was found to be non-cytotoxic and prevented the scattering of the cells after delivery. The gelatin hydrogel constructs kept the carried cells viable; also, the porosity and permeability were adequate for a perfusion system. Cell proliferation was better under perfusion culture than under static culture. When human umbilical vein endothelial cells were seeded into the constructs, we demonstrated that they stably formed an even coverage on the surface of the hydrogel filaments, serving as a preliminary microvasculature network. We concluded that this method provides a viable solution for cell seeding, oxygen delivery, and mass transfer in large three-dimensional (3-D) tissue engineering. Furthermore, it has the potential for being a workhorse in studies involving 3-D cell cultures and tissue engineering.

Original languageEnglish
Pages (from-to)912-920
Number of pages9
JournalActa Biomaterialia
Issue number2
Publication statusPublished - Feb 2014


  • Cell culture
  • Hydrogel
  • Scaffold
  • Three-dimensional
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology


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