Fabrication of biofunctional oxide layer with potential self-antibacterial performance for biomedical applications

Yung Chieh Cho, Chung Chih Tseng, Keng Liang Ou, Liang Yo Yang, Wen Chien Lan, Bai Hung Huang, Chia Chien Hsieh, Chien Chuan Wang, Mao Suan Huang

Research output: Contribution to journalArticlepeer-review


In the present study, field-emission scanning electron microscopy, Auger electron spectroscopy, surface roughness instrument, and transmission electron microscopy were performed to investigate a potential self-antibacterial oxide layer on the titanium (Ti) surface formed by the anodization with hydrogen fluoride (HF) acid pretreatment through cathodization. The in vitro antibacterial properties were determined using the Gram-positive bacterium Staphylococcus aureus (S. aureus) and Gram-negative bacterium Escherichia coli (E. coli) according to JIS Z2801: 2010 specification. Analytical results showed that the topography with a flat nano-porous structure was formed on the anodized Ti specimen with 0.5% HF pretreatment. The pretreatment with a concentration of 0.5% HF caused a thicker Ti dioxide layer (∼350 nm) formation on the Ti surface after anodization. Moreover, the antibacterial rate also proved that the anodized Ti specimen with 0.5% HF pretreatment had the potential to inhibit both S. aureus (75.1 ± 3.5%) and E. coli (90.5 ± 1.6%) growth (*p < 0.05). Hence, the anodization with HF pretreatment through cathodization approach can be used to fabricate a biofunctional surface with self-antibacterial performance that is required for biomedical Ti implant applications.

Original languageEnglish
Article number112929
JournalMaterials Characterization
Publication statusPublished - Jul 2023


  • Antibacterial rate
  • Biofunctional surface
  • Hydrogen fluoride
  • Surface modification
  • Titanium implant

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


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