TY - JOUR
T1 - Expressions of chemokines and their receptors in the brain after heat stroke-induced cortical damage
AU - Lin, Yuh Feng
AU - Liu, Tsung Ta
AU - Hu, Chou Hui
AU - Chen, Chun Chi
AU - Wang, Jia Yi
N1 - Funding Information:
This work was supported by grants from Taipei Medical University - Shuang Ho Hospital ( 102TMU-SHH-01-2 to YFL) and the Ministry of Science and Technology (MOST 104-2923-B-038-001-MY3 to JYW). Formal approval to conduct the experiments described was obtained from the Animal Use and Care Committee of the Taipei Medical University. There are no conflicts of interests associated with this study.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/5/15
Y1 - 2018/5/15
N2 - Despite growing evidence that cytokines and chemokines are expressed in humans and rats after heat stress, the cellular mechanisms underlying the effects on the brain after heatstroke (HS) are not fully understood. In this study, we observed time course changes of chemokines in rat brain tissues and elucidated what kinds of cortical cells were affected after HS. Male SD rats were anesthetized and randomly separated into two groups as follows: (a) normothermic sham and (b) HS rats. Rats were sacrificed at different time points (0, 1, 3, 6, and 12. h after heat exposure, n = 5 in each group) to the end of the experiment in order to extract the mRNA/proteins of cortical tissues. Cerebrospinal fluid (CSF) of sham and HS rats was also collected before sacrifice. In the HS group, an elevated body temperature (Tco. >. 40. °C) and abnormality of cortical cells (e.g., pyknotic nuclei) were observed. When compared to the sham group, expression levels of either mRNAs or proteins of chemokines and their receptors (including CXCL1, MIP2, MCP1, CXCR1, CXCR2, and CCR2) peaked at different time points after heat exposure. We also found that CXCR2 was expressed in the cortex of rat brain and was colocalized with neurons and microglia after HS. Hence, MCP1, MIP2, and CXCR2 might play important roles in the brain after HS, possibly indicating a new direction for treating HS.
AB - Despite growing evidence that cytokines and chemokines are expressed in humans and rats after heat stress, the cellular mechanisms underlying the effects on the brain after heatstroke (HS) are not fully understood. In this study, we observed time course changes of chemokines in rat brain tissues and elucidated what kinds of cortical cells were affected after HS. Male SD rats were anesthetized and randomly separated into two groups as follows: (a) normothermic sham and (b) HS rats. Rats were sacrificed at different time points (0, 1, 3, 6, and 12. h after heat exposure, n = 5 in each group) to the end of the experiment in order to extract the mRNA/proteins of cortical tissues. Cerebrospinal fluid (CSF) of sham and HS rats was also collected before sacrifice. In the HS group, an elevated body temperature (Tco. >. 40. °C) and abnormality of cortical cells (e.g., pyknotic nuclei) were observed. When compared to the sham group, expression levels of either mRNAs or proteins of chemokines and their receptors (including CXCL1, MIP2, MCP1, CXCR1, CXCR2, and CCR2) peaked at different time points after heat exposure. We also found that CXCR2 was expressed in the cortex of rat brain and was colocalized with neurons and microglia after HS. Hence, MCP1, MIP2, and CXCR2 might play important roles in the brain after HS, possibly indicating a new direction for treating HS.
KW - Acute brain damage
KW - Chemokines
KW - Heat stroke
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U2 - 10.1016/j.jneuroim.2018.01.014
DO - 10.1016/j.jneuroim.2018.01.014
M3 - Article
AN - SCOPUS:85041237233
SN - 0165-5728
VL - 318
SP - 15
EP - 20
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
ER -