Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion

W. C. Liao; C. H. Chen; C. H. Liu; M. J. Huang; C. W. Chen; J. S. Hung; C. H. Chou; C. H. Chen; M. I. Che; H. M. Chang; C. T. Lan; H. C. Huang; G. F. Tseng; M. K. Shyu; M. C. Huang

doi:10.1016/j.placenta.2012.08.007

Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion

W. C. Liao, C. H. Chen, C. H. Liu, M. J. Huang, C. W. Chen, J. S. Hung, C. H. Chou, C. H. Chen, M. I. Che, H. M. Chang, C. T. Lan, H. C. Huang, G. F. Tseng, M. K. Shyu, M. C. Huang

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Extravillus trophoblast (EVT) invasion plays a critical role in placental development. Integrins bind to extracellular matrix (ECM) proteins to mediate EVT cell adhesion, migration, and invasion. Changes in O-glycans on β1-integrin have been found to regulate cancer cell behavior. We hypothesize that O-glycosyltransferases can regulate EVT invasion through modulating the glycosylation and function of β1-integrin. Here, we found that the GALNT1 and GALNT2 mRNA were highly expressed in HTR8/SVneo and first trimester EVT cells. Immunohistochemstry and immunofluorescence staining showed that GALNT2 was expressed in subpopulations of EVT cells in deciduas, but not in syncytiotrophoblasts and cytotrophoblasts of placental villi. The percentage of GALNT2-positive EVT cells increased with gestational ages. Overexpression of GALNT2 in HTR8/SVneo cells significantly enhanced cell-collagen IV adhesion, but suppressed cell migration and invasion. Notably, we found that GALNT2 increased the expression of Tn antigen (GalNAc-Ser/Thr) on β1-integrin as revealed by Vicia Villosa agglutinin (VVA) binding. Furthermore, GALNT2 suppressed the phosphorylation of focal adhesion kinase (FAK), a crucial downstream signaling molecule of β1-integrin. Our findings suggest that GALNT2 is a critical initiating enzyme of O-glycosylation for regulating EVT invasion.

Original language	English
Pages (from-to)	1005-1011
Number of pages	7
Journal	Placenta
Volume	33
Issue number	12
DOIs	https://doi.org/10.1016/j.placenta.2012.08.007
Publication status	Published - Dec 2012

Keywords

EVT
GALNT
Integrin
Invasion
Mucin
O-glycans

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.placenta.2012.08.007

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Liao, W. C., Chen, C. H., Liu, C. H., Huang, M. J., Chen, C. W., Hung, J. S., Chou, C. H., Chen, C. H., Che, M. I., Chang, H. M., Lan, C. T., Huang, H. C., Tseng, G. F., Shyu, M. K., & Huang, M. C. (2012). Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion. Placenta, 33(12), 1005-1011. https://doi.org/10.1016/j.placenta.2012.08.007

@article{a8edeb20b7c94044954f1deb0b7aa0e6,

title = "Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion",

abstract = "Extravillus trophoblast (EVT) invasion plays a critical role in placental development. Integrins bind to extracellular matrix (ECM) proteins to mediate EVT cell adhesion, migration, and invasion. Changes in O-glycans on β1-integrin have been found to regulate cancer cell behavior. We hypothesize that O-glycosyltransferases can regulate EVT invasion through modulating the glycosylation and function of β1-integrin. Here, we found that the GALNT1 and GALNT2 mRNA were highly expressed in HTR8/SVneo and first trimester EVT cells. Immunohistochemstry and immunofluorescence staining showed that GALNT2 was expressed in subpopulations of EVT cells in deciduas, but not in syncytiotrophoblasts and cytotrophoblasts of placental villi. The percentage of GALNT2-positive EVT cells increased with gestational ages. Overexpression of GALNT2 in HTR8/SVneo cells significantly enhanced cell-collagen IV adhesion, but suppressed cell migration and invasion. Notably, we found that GALNT2 increased the expression of Tn antigen (GalNAc-Ser/Thr) on β1-integrin as revealed by Vicia Villosa agglutinin (VVA) binding. Furthermore, GALNT2 suppressed the phosphorylation of focal adhesion kinase (FAK), a crucial downstream signaling molecule of β1-integrin. Our findings suggest that GALNT2 is a critical initiating enzyme of O-glycosylation for regulating EVT invasion.",

keywords = "EVT, GALNT, Integrin, Invasion, Mucin, O-glycans",

author = "Liao, {W. C.} and Chen, {C. H.} and Liu, {C. H.} and Huang, {M. J.} and Chen, {C. W.} and Hung, {J. S.} and Chou, {C. H.} and Chen, {C. H.} and Che, {M. I.} and Chang, {H. M.} and Lan, {C. T.} and Huang, {H. C.} and Tseng, {G. F.} and Shyu, {M. K.} and Huang, {M. C.}",

note = "Funding Information: This study was supported by the grants from the National Science Council NSC101-2314-B-002-058 (to Dr. Hung JS), NSC98-2320-B-002-032-MY3 and NSC101-2320-B-002-007-MY3 (to Dr. Huang MC), NSC101-2320-B-040-003 (to Dr. Liao WC) and NSC96-2314-B-002-089-MY3 (to Dr. Shyu MK), as well as from National Taiwan University Hospital NTUH100S-1625 (to Dr. Shyu MK).",

year = "2012",

month = dec,

doi = "10.1016/j.placenta.2012.08.007",

language = "English",

volume = "33",

pages = "1005--1011",

journal = "Placenta",

issn = "0143-4004",

publisher = "W.B. Saunders Ltd",

number = "12",

}

TY - JOUR

T1 - Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion

AU - Liao, W. C.

AU - Chen, C. H.

AU - Liu, C. H.

AU - Huang, M. J.

AU - Chen, C. W.

AU - Hung, J. S.

AU - Chou, C. H.

AU - Chen, C. H.

AU - Che, M. I.

AU - Chang, H. M.

AU - Lan, C. T.

AU - Huang, H. C.

AU - Tseng, G. F.

AU - Shyu, M. K.

AU - Huang, M. C.

N1 - Funding Information: This study was supported by the grants from the National Science Council NSC101-2314-B-002-058 (to Dr. Hung JS), NSC98-2320-B-002-032-MY3 and NSC101-2320-B-002-007-MY3 (to Dr. Huang MC), NSC101-2320-B-040-003 (to Dr. Liao WC) and NSC96-2314-B-002-089-MY3 (to Dr. Shyu MK), as well as from National Taiwan University Hospital NTUH100S-1625 (to Dr. Shyu MK).

PY - 2012/12

Y1 - 2012/12

N2 - Extravillus trophoblast (EVT) invasion plays a critical role in placental development. Integrins bind to extracellular matrix (ECM) proteins to mediate EVT cell adhesion, migration, and invasion. Changes in O-glycans on β1-integrin have been found to regulate cancer cell behavior. We hypothesize that O-glycosyltransferases can regulate EVT invasion through modulating the glycosylation and function of β1-integrin. Here, we found that the GALNT1 and GALNT2 mRNA were highly expressed in HTR8/SVneo and first trimester EVT cells. Immunohistochemstry and immunofluorescence staining showed that GALNT2 was expressed in subpopulations of EVT cells in deciduas, but not in syncytiotrophoblasts and cytotrophoblasts of placental villi. The percentage of GALNT2-positive EVT cells increased with gestational ages. Overexpression of GALNT2 in HTR8/SVneo cells significantly enhanced cell-collagen IV adhesion, but suppressed cell migration and invasion. Notably, we found that GALNT2 increased the expression of Tn antigen (GalNAc-Ser/Thr) on β1-integrin as revealed by Vicia Villosa agglutinin (VVA) binding. Furthermore, GALNT2 suppressed the phosphorylation of focal adhesion kinase (FAK), a crucial downstream signaling molecule of β1-integrin. Our findings suggest that GALNT2 is a critical initiating enzyme of O-glycosylation for regulating EVT invasion.

AB - Extravillus trophoblast (EVT) invasion plays a critical role in placental development. Integrins bind to extracellular matrix (ECM) proteins to mediate EVT cell adhesion, migration, and invasion. Changes in O-glycans on β1-integrin have been found to regulate cancer cell behavior. We hypothesize that O-glycosyltransferases can regulate EVT invasion through modulating the glycosylation and function of β1-integrin. Here, we found that the GALNT1 and GALNT2 mRNA were highly expressed in HTR8/SVneo and first trimester EVT cells. Immunohistochemstry and immunofluorescence staining showed that GALNT2 was expressed in subpopulations of EVT cells in deciduas, but not in syncytiotrophoblasts and cytotrophoblasts of placental villi. The percentage of GALNT2-positive EVT cells increased with gestational ages. Overexpression of GALNT2 in HTR8/SVneo cells significantly enhanced cell-collagen IV adhesion, but suppressed cell migration and invasion. Notably, we found that GALNT2 increased the expression of Tn antigen (GalNAc-Ser/Thr) on β1-integrin as revealed by Vicia Villosa agglutinin (VVA) binding. Furthermore, GALNT2 suppressed the phosphorylation of focal adhesion kinase (FAK), a crucial downstream signaling molecule of β1-integrin. Our findings suggest that GALNT2 is a critical initiating enzyme of O-glycosylation for regulating EVT invasion.

KW - EVT

KW - GALNT

KW - Integrin

KW - Invasion

KW - Mucin

KW - O-glycans

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UR - http://www.scopus.com/inward/citedby.url?scp=84869504035&partnerID=8YFLogxK

U2 - 10.1016/j.placenta.2012.08.007

DO - 10.1016/j.placenta.2012.08.007

M3 - Article

C2 - 23117232

AN - SCOPUS:84869504035

SN - 0143-4004

VL - 33

SP - 1005

EP - 1011

JO - Placenta

JF - Placenta

IS - 12

ER -

Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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