Expression of caveolin-1 in the early phase of beta-TCP implanted in dog mandible

Cherng Tzeh Chou, Ujjal K. Bhawal, Nobuyuki Watanabe, Noboru Kuboyama, Wei Jen Chang, Sheng Yang Lee, Yoshimitsu Abiko

Research output: Contribution to journalArticlepeer-review


Caveolin is an essential and signature protein of caveolae. Caveolin-1 participates in signal transduction processes by acting as a scaffolding protein that concentrates, organizes and functional regulates signalling molecules within caveolar membranes. Beta-tricalcium phosphate (β-TCP) has been widely used for scaffold in tissue engineering due to its high biodegradability, osteoconductivity, easy manipulation, and lack of histotoxicity. To better understand the role of caveolin-1 in bone homeostasis and response to β-TCP scaffold, β-TCP was implanted into the dog mandible defects in beagle dogs, and gene expression profiles were examined focused on the molecular components involved in caveolin-1 regulation. Here we showed the quantitative imageology analysis characterized using in vivo micro-computed tomography (CT) images at 4 and 7 days after β-TCP implanted in dog mandibles. The bone reformation by using the β-TCP scaffolds began within 4 days of surgery, and was healing well at 7 days after surgery. Higher mRNA level of caveolin-1 was observed in β-TCP-implanted Beagle dog mandibles compared with controls at day 4 and day 7 post-surgery. The enhancement of caveolin-1 by β-TCP was further confirmed by immunohistochemistry and immunofluorescence analysis. We further revealed increased Smad7 and Phospho Stat3 expression in β-TCP-implanted specimens. Taken together, these results suggest that the enhancement of caveolin-1 play an important role in accelerating bone formation by β-TCP.

Original languageEnglish
Pages (from-to)804-812
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume101 B
Issue number5
Publication statusPublished - Jul 2013


  • bone regeneration
  • caveolin-1
  • β-TCP scaffold

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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