Expression and characterization of two STAT isoforms from Sf9 cells

Maw Sheng Yeh; Chia Hsiung Cheng; Chih Ming Chou; Ya Li Hsu; Cheng Ying Chu; Gen Der Chen; Shui Tsung Chen; Guang Chao Chen; Chang Jen Huang

doi:10.1016/j.dci.2007.12.001

Expression and characterization of two STAT isoforms from Sf9 cells

Maw Sheng Yeh, Chia Hsiung Cheng, Chih Ming Chou, Ya Li Hsu, Cheng Ying Chu, Gen Der Chen, Shui Tsung Chen, Guang Chao Chen, Chang Jen Huang

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.

Original language	English
Pages (from-to)	814-824
Number of pages	11
Journal	Developmental and Comparative Immunology
Volume	32
Issue number	7
DOIs	https://doi.org/10.1016/j.dci.2007.12.001
Publication status	Published - 2008

Keywords

Baculovirus
DNA binding
Insect Sf9 cells
STAT

ASJC Scopus subject areas

Immunology
Developmental Biology

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10.1016/j.dci.2007.12.001

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abstract = "In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.",

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T1 - Expression and characterization of two STAT isoforms from Sf9 cells

AU - Yeh, Maw Sheng

AU - Cheng, Chia Hsiung

AU - Chou, Chih Ming

AU - Hsu, Ya Li

AU - Chu, Cheng Ying

AU - Chen, Gen Der

AU - Chen, Shui Tsung

AU - Chen, Guang Chao

AU - Huang, Chang Jen

PY - 2008

Y1 - 2008

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AB - In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.

KW - Baculovirus

KW - DNA binding

KW - Insect Sf9 cells

KW - STAT

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JO - Developmental and Comparative Immunology

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Expression and characterization of two STAT isoforms from Sf9 cells

Abstract

Keywords

ASJC Scopus subject areas

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