Abstract
In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
Original language | English |
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Pages (from-to) | 814-824 |
Number of pages | 11 |
Journal | Developmental and Comparative Immunology |
Volume | 32 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2008 |
Keywords
- Baculovirus
- DNA binding
- Insect Sf9 cells
- STAT
ASJC Scopus subject areas
- Developmental Biology
- Immunology