TY - JOUR
T1 - Exposure to residential indoor air induces heritable DNA mutations in mice
AU - Zhou, Yin
AU - Liu, Yunru
AU - Qiu, Wen
AU - Zeng, Jinyan
AU - Chen, Xiaodong
AU - Zhou, Hanzhi
AU - Li, Aiping
AU - Zhou, Jianwei
N1 - Funding Information:
Received 30 April 2009; accepted 12 June 2009. Yin Zhou, Yunru Liu, and Wen Qiu contributed equally to this work. This work was supported partially by the National Natural Science Foundation of China (30872085). Address correspondence to Prof. Jianwei Zhou, MD, PhD, Department of Molecular Cell Biology and Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China. E-mail: [email protected] rodents, which frequently involve specific loci and dominant lethal tests, are examined (Russell & Matter, 1980). These approaches have two disadvantages. First, spontaneous mutation rates at the loci monitored are low. Second, doses of radiation or chemical mutagens administered to F0 animals need to be high in order to induce statistically detectable increases in mutant phenotypes in their F1 offspring (Somers, 2006). Interpretation of results from this experimental protocol has limitations because most occupational and environmental exposures are likely to be chronic, low doses of complex mixtures. Therefore, a key challenge is to evaluate effects of chronic, low-dose exposures, such as those produced by air pollutants released during indoor home improvements. Thus, exposure of humans undertaking home improvement activities can be examined for genotoxicity directly in the parents as well as in the subsequent generation by studying their children.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Exposure to the mixed indoor air pollutants released from synthetic materials and chemical products poses a serious public health problem, but little evidence has been provided to clarify whether such pollutants at environmentally relevant concentrations produce inheritable germline mutations. In the present study, mice were exposed to samples of indoor air from a newly decorated apartment bedroom. Results showed expanded simple tandem repeat (ESTR) mutations occurring in the germline of control and exposed parents, which were also detected in their offspring using three probes, Ms6-hm, Hm-2, and MMS10. Data indicated that mice being exposed to indoor air triggered a significant increase in frequency of ESTR mutations, which may be due primarily to a rise in mutations inherited through the paternal germline. These results suggest that exposure to a mixture of pollutants in indoor air obtained from an apartment in China induced ESTR mutations. Thus, humans exposed to polluted indoor apartment air in China may be at risk for developing germline mutations.
AB - Exposure to the mixed indoor air pollutants released from synthetic materials and chemical products poses a serious public health problem, but little evidence has been provided to clarify whether such pollutants at environmentally relevant concentrations produce inheritable germline mutations. In the present study, mice were exposed to samples of indoor air from a newly decorated apartment bedroom. Results showed expanded simple tandem repeat (ESTR) mutations occurring in the germline of control and exposed parents, which were also detected in their offspring using three probes, Ms6-hm, Hm-2, and MMS10. Data indicated that mice being exposed to indoor air triggered a significant increase in frequency of ESTR mutations, which may be due primarily to a rise in mutations inherited through the paternal germline. These results suggest that exposure to a mixture of pollutants in indoor air obtained from an apartment in China induced ESTR mutations. Thus, humans exposed to polluted indoor apartment air in China may be at risk for developing germline mutations.
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U2 - 10.1080/15287390903232442
DO - 10.1080/15287390903232442
M3 - Article
C2 - 20077230
AN - SCOPUS:75149194887
SN - 1528-7394
VL - 72
SP - 1561
EP - 1566
JO - Journal of Toxicology and Environmental Health - Part A: Current Issues
JF - Journal of Toxicology and Environmental Health - Part A: Current Issues
IS - 24
ER -
