Evodiamine represses hypoxia-induced inflammatory proteins expression and hypoxia-inducible factor 1α accumulation in RAW264.7

Yi Nan Liu, Shiow Lin Pan, Cho Hwa Liao, Der Yi Huang, Jih Hwa Guh, Chieh Yu Peng, Ya Ling Chang, Che Ming Teng

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60 Citations (Scopus)


Inflammation and low oxygen diffusion are recognized characteristics of cardiovascular diseases such as atherosclerosis. Evodiamine, extracted from the traditional Chinese herb, Evodia rutaecarpa, is a bioactive anti-inflammatory alkaloid. The objective of this study was to investigate whether evodiamine could repress hypoxia-induced inflammatory response. We showed that evodiamine repressed not only COX-2 and iNOS expression but also prostaglandin E2 release in a concentration-dependent manner under hypoxic conditions. Furthermore, our studies indicated that COX-2 mRNA was inhibited by evodiamine, implying that transcriptional activity is involved in the mechanistic pathway. It is striking that hypoxia-inducible factor 1α (HIF-1α) inhibitor, camptothecin, suppressed hypoxia-induced COX-2 expression rather than pyrrolidine dithiocarbamate, a nuclear factor κB inhibitor. In addition, our studies have confirmed that evodiamine inhibited HIF-1α, which accounted for the transcriptional activity of COX-2, rather than nuclear factor κB in RAW264.7 cells. Finally, evodiamine did not affect either the level of HIF-1α mRNA or the degradation rate of HIF-1α protein, but it regulated the translational process of HIF-1α. We found that hypoxia-evoked phosphorylation of Akt and p70S6K was blocked after evodiamine treatment, in addition to the inhibition of phosphorylation of 4E-BP. These results suggest that the mechanism of repression of hypoxia-induced COX-2 expression by evodiamine is through the inhibition of HIF-1α at the translational level and is primarily mediated via dephosphorylation of Akt and p70S6K. Therefore, evodiamine could be an effective therapeutic agent against inflammatory diseases involving hypoxia.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
Issue number3
Publication statusPublished - Sept 2009
Externally publishedYes


  • Macrophage
  • p70S6K
  • Translation

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine


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