Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay

Kuo Ching Yang, Alexander Chu, Chao Sheng Liao, Yu Min Lin, Gen Min Wang

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Aim: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. Methods: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore). Results: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference. Conclusion: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.

Original languageEnglish
Pages (from-to)7029-7032
Number of pages4
JournalWorld Journal of Gastroenterology
Issue number43
Publication statusPublished - Nov 21 2006


  • Enzyme-linked immunosorbent assay
  • Gastric cancer
  • H pylori
  • Immunoblotting
  • Western blot

ASJC Scopus subject areas

  • Gastroenterology


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