Evaluation of porous bacterial cellulose produced from foam templating with different additives and its application in 3D cell culture

Shin Ping Lin, Stephanie Singajaya, Tsui Yun Lo, Shella Permatasari Santoso, Hsien Yi Hsu, Kuan Chen Cheng

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering.

Original languageEnglish
Article number123680
JournalInternational Journal of Biological Macromolecules
Volume234
DOIs
Publication statusPublished - Apr 2023

Keywords

  • 3D cell culture
  • Bacterial cellulose
  • Foam templating
  • Tissue scaffold

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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