Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

Tsi Shu Huang; Yung Ching Liu; Wen Kuei Huang; Chii Shiang Chen; Hui Zin Tu; Deh Lin Cheng

Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

Tsi Shu Huang, Yung Ching Liu, Wen Kuei Huang, Chii Shiang Chen, Hui Zin Tu, Deh Lin Cheng

Research output: Contribution to journal › Review article › peer-review

Abstract

Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

Original language	English
Pages (from-to)	530-535
Number of pages	6
Journal	Journal of the Formosan Medical Association
Volume	95
Issue number	7
Publication status	Published - Jul 1996
Externally published	Yes

Keywords

BACTEC TB system
Mycobacteria
Polymerase chain reaction
Restriction enzyme analysis

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{fc63c7a807a54bec8d096be4124b373b,

title = "Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles",

abstract = "Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.",

keywords = "BACTEC TB system, Mycobacteria, Polymerase chain reaction, Restriction enzyme analysis",

author = "Huang, {Tsi Shu} and Liu, {Yung Ching} and Huang, {Wen Kuei} and Chen, {Chii Shiang} and Tu, {Hui Zin} and Cheng, {Deh Lin}",

year = "1996",

month = jul,

language = "English",

volume = "95",

pages = "530--535",

journal = "Journal of the Formosan Medical Association",

issn = "0929-6646",

publisher = "Elsevier B.V.",

number = "7",

}

TY - JOUR

T1 - Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

AU - Huang, Tsi Shu

AU - Liu, Yung Ching

AU - Huang, Wen Kuei

AU - Chen, Chii Shiang

AU - Tu, Hui Zin

AU - Cheng, Deh Lin

PY - 1996/7

Y1 - 1996/7

N2 - Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

AB - Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

KW - BACTEC TB system

KW - Mycobacteria

KW - Polymerase chain reaction

KW - Restriction enzyme analysis

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M3 - Review article

C2 - 8840754

AN - SCOPUS:0030184703

SN - 0929-6646

VL - 95

SP - 530

EP - 535

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

IS - 7

ER -

Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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