Abstract
We propose the adjuvant effects of phospholipid liposome compositions using intranasal inoculation of a liposomal-Newcastle disease virus (NDV) vaccine in chickens. The immunogenicity of three liposome formulations was determined in chickens using the hemagglutination-inhibition (HI) test, nasal secretory immunoglobulin A and serum immunoglobulin A (IgG) antibody titers using the enzyme-linked immunosorbent assay. The immune response against NDV antigens was determined after immunization with neutral charged liposomes composed of egg phosphatidylcholine (EPC) (60 μmol), cholesterol (Chol) (15 μmol), and EPC-liposomes (EPC-Lip), which elicited strong systemic (serum) and local (nasal) humoral responses. However, the intranasal administration with cationic charged liposomes composed of EPC (30 μmol), stearylamine (SA) (15 μmol), Chol (15 μmol), and SA-liposomes (SA-Lip) induced poor humoral immune responses. Only the vaccine formulated with anionic charged liposomes composed of EPC (30 μmol), dipalmitoylphosphatidylserine (15 μmol), Chol (15 μmol), and phosphatidylserine-liposomes (PS-Lip) elicited the highest titers of HI antibodies. These are the first results to suggest that antigen delivery using EPCLip is very useful in enhancing antibody production at the mucosal site and in serum.
Original language | English |
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Pages (from-to) | 621-625 |
Number of pages | 5 |
Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
Volume | 91 |
Issue number | 2 |
DOIs | |
Publication status | Published - Nov 2009 |
Keywords
- Adjuvant
- Intranasal delivery
- Liposomal vaccine
- Newcastle disease virus
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering