Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system

  • Kuo Hsien Hsieh
  • , Jie Jen Lee
  • , Lin Wen Lee
  • , Joen Rong Sheu
  • , Su Tzy Hwang
  • , Duen Suey Chou

Research output: Contribution to journalArticlepeer-review

Abstract

A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.

Original languageEnglish
Pages (from-to)281-288+378
JournalJournal of Food and Drug Analysis
Volume19
Issue number3
Publication statusPublished - Sept 2011

Keywords

  • Arachidonic acid
  • Carbon-centered radical
  • Electron spin resonance
  • Hydroxyl radical
  • Platelets

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

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