Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system

Kuo Hsien Hsieh; Jie Jen Lee; Lin Wen Lee; Joen Rong Sheu; Su Tzy Hwang; Duen Suey Chou

Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system

Kuo Hsien Hsieh, Jie Jen Lee, Lin Wen Lee, Joen Rong Sheu, Su Tzy Hwang, Duen Suey Chou

Research output: Contribution to journal › Article › peer-review

Abstract

A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.

Original language	English
Pages (from-to)	281-288+378
Journal	Journal of Food and Drug Analysis
Volume	19
Issue number	3
Publication status	Published - Sept 2011

Keywords

Arachidonic acid
Carbon-centered radical
Electron spin resonance
Hydroxyl radical
Platelets

ASJC Scopus subject areas

Food Science
Pharmacology

Cite this

@article{f797965201314992ac17226b312f987d,

title = "Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system",

abstract = "A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.",

keywords = "Arachidonic acid, Carbon-centered radical, Electron spin resonance, Hydroxyl radical, Platelets",

author = "Hsieh, {Kuo Hsien} and Lee, {Jie Jen} and Lee, {Lin Wen} and Sheu, {Joen Rong} and Hwang, {Su Tzy} and Chou, {Duen Suey}",

year = "2011",

month = sep,

language = "English",

volume = "19",

pages = "281--288+378",

journal = "Journal of Food and Drug Analysis",

issn = "1021-9498",

publisher = "National Laboratories of Foods and Drugs",

number = "3",

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TY - JOUR

T1 - Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system

AU - Hsieh, Kuo Hsien

AU - Lee, Jie Jen

AU - Lee, Lin Wen

AU - Sheu, Joen Rong

AU - Hwang, Su Tzy

AU - Chou, Duen Suey

PY - 2011/9

Y1 - 2011/9

N2 - A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.

AB - A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.

KW - Arachidonic acid

KW - Carbon-centered radical

KW - Electron spin resonance

KW - Hydroxyl radical

KW - Platelets

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UR - http://www.scopus.com/inward/citedby.url?scp=83455179702&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:83455179702

SN - 1021-9498

VL - 19

SP - 281-288+378

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

IS - 3

ER -

Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system

Abstract

Keywords

ASJC Scopus subject areas

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