Evaluating skin cancer chemopreventive potential of water extract of Syzygium samarangense leaves through activation of the Nrf2-mediated cellular defense system

Zheng Yuan Su, Yen Fan Chan, Yen Chen Tung, Ping Hua Sung, Pei Chun Chen, Zi Han Lin, Tien Yuan Wu

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Oxidative stress has been demonstrated to cause gene mutations which may increase the morbidity of cancer; however, many phytochemicals may increase cellular antioxidant capacity for cancer chemoprevention in humans. Here, we evaluated skin cancer chemopreventive potential of Syzygium samarangense (wax apple) leaf water extract (WAL-WE) by activating Nrf2-mediated cellular antioxidant system. The results showed that WAL-WE at 100 and 250 μg/mL exhibited Nrf2-ARE pathway induction in the human HepG2-C8 cells with stably transfected ARE-luciferase plasmids. WAL-WE (0.25–2.0 μg/mL) also effectively inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse epidermal JB6 P+ cell transformation. WAL-WE increased mRNA and protein expressions of Nrf2 and Nrf2 downstream antioxidant and detoxification enzymes, such as HO-1, NQO1, and UGT1A, in HepG2-C8 and JB6 P+ cells. Moreover, WAL-WE was fractionated into 25 parts by applying a silica gel column chromatography. Among them, fraction 15 (eluted by ethyl acetate/methanol = 80:20) with higher yield showed the best Nrf2-ARE induction in HepG2-C8 cells, but the active components are needed to be further elucidated. These results suggest that WAL-WE may be a potential skin cancer chemopreventive agent through activation of the Nrf2 cellular defense pathway.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
JournalSouth African Journal of Botany
Volume137
DOIs
Publication statusPublished - Mar 2021
Externally publishedYes

Keywords

  • Cancer chemoprevention
  • Cellular antioxidant ability
  • Nrf2
  • Silica gel column chromatography
  • Syzygium samarangense

ASJC Scopus subject areas

  • Plant Science

Fingerprint

Dive into the research topics of 'Evaluating skin cancer chemopreventive potential of water extract of Syzygium samarangense leaves through activation of the Nrf2-mediated cellular defense system'. Together they form a unique fingerprint.

Cite this