TY - JOUR
T1 - Evaluating skin cancer chemopreventive potential of water extract of Syzygium samarangense leaves through activation of the Nrf2-mediated cellular defense system
AU - Su, Zheng Yuan
AU - Chan, Yen Fan
AU - Tung, Yen Chen
AU - Sung, Ping Hua
AU - Chen, Pei Chun
AU - Lin, Zi Han
AU - Wu, Tien Yuan
N1 - Funding Information:
This research was supported by MOST 105-2311-B-033-002 from Ministry of Science and Technology. We thank all the members in Dr. Su's lab for their helpful discussion of this work.
Funding Information:
This research was supported by MOST 105-2311-B-033-002 from Ministry of Science and Technology. We thank all the members in Dr. Su's lab for their helpful discussion of this work.
Publisher Copyright:
© 2020 SAAB
PY - 2021/3
Y1 - 2021/3
N2 - Oxidative stress has been demonstrated to cause gene mutations which may increase the morbidity of cancer; however, many phytochemicals may increase cellular antioxidant capacity for cancer chemoprevention in humans. Here, we evaluated skin cancer chemopreventive potential of Syzygium samarangense (wax apple) leaf water extract (WAL-WE) by activating Nrf2-mediated cellular antioxidant system. The results showed that WAL-WE at 100 and 250 μg/mL exhibited Nrf2-ARE pathway induction in the human HepG2-C8 cells with stably transfected ARE-luciferase plasmids. WAL-WE (0.25–2.0 μg/mL) also effectively inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse epidermal JB6 P+ cell transformation. WAL-WE increased mRNA and protein expressions of Nrf2 and Nrf2 downstream antioxidant and detoxification enzymes, such as HO-1, NQO1, and UGT1A, in HepG2-C8 and JB6 P+ cells. Moreover, WAL-WE was fractionated into 25 parts by applying a silica gel column chromatography. Among them, fraction 15 (eluted by ethyl acetate/methanol = 80:20) with higher yield showed the best Nrf2-ARE induction in HepG2-C8 cells, but the active components are needed to be further elucidated. These results suggest that WAL-WE may be a potential skin cancer chemopreventive agent through activation of the Nrf2 cellular defense pathway.
AB - Oxidative stress has been demonstrated to cause gene mutations which may increase the morbidity of cancer; however, many phytochemicals may increase cellular antioxidant capacity for cancer chemoprevention in humans. Here, we evaluated skin cancer chemopreventive potential of Syzygium samarangense (wax apple) leaf water extract (WAL-WE) by activating Nrf2-mediated cellular antioxidant system. The results showed that WAL-WE at 100 and 250 μg/mL exhibited Nrf2-ARE pathway induction in the human HepG2-C8 cells with stably transfected ARE-luciferase plasmids. WAL-WE (0.25–2.0 μg/mL) also effectively inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse epidermal JB6 P+ cell transformation. WAL-WE increased mRNA and protein expressions of Nrf2 and Nrf2 downstream antioxidant and detoxification enzymes, such as HO-1, NQO1, and UGT1A, in HepG2-C8 and JB6 P+ cells. Moreover, WAL-WE was fractionated into 25 parts by applying a silica gel column chromatography. Among them, fraction 15 (eluted by ethyl acetate/methanol = 80:20) with higher yield showed the best Nrf2-ARE induction in HepG2-C8 cells, but the active components are needed to be further elucidated. These results suggest that WAL-WE may be a potential skin cancer chemopreventive agent through activation of the Nrf2 cellular defense pathway.
KW - Cancer chemoprevention
KW - Cellular antioxidant ability
KW - Nrf2
KW - Silica gel column chromatography
KW - Syzygium samarangense
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U2 - 10.1016/j.sajb.2020.10.029
DO - 10.1016/j.sajb.2020.10.029
M3 - Article
AN - SCOPUS:85096222609
SN - 0254-6299
VL - 137
SP - 303
EP - 310
JO - South African Journal of Botany
JF - South African Journal of Botany
ER -
