TY - JOUR
T1 - Enrichment and depletion of hela topoisomerase I recognition sites among specific types of DNA elements
AU - Perez-Stable, Carlos
AU - Shen, Chein Celia
AU - Shen, Che Kun
PY - 1988/8/25
Y1 - 1988/8/25
N2 - The SDS-induced nicking of DNA helix by Hela topoisomerase I in vitro haa been studied by using 2.9 kb of cloned human DNA as the substrate. The frequency of nicking is increased from 1/23 (nick/nt) to 1/19 (nick/nt) when camptothecin is present in the nicking reaction. The cytotoxic drug also induces DNA nicks without the addition of SDS. Although the consensus built from DNA sequences from -20 to +20 of more tham one hundred of the nicking sites only shows a preference for T at position -1, the distributions of the topoisomerae I-cleavable sites among different categories of specific DNA sequences are apparently non- random. Long stretches of tandem (CA), A, or T residues, and the GC-rich promoter region of C globin gene are all refractory to the nicking reaction. Howevar, the nicking frequencies of short direct repeats flanking different Alu type sequences are as high as 1/6 (nick/nt). Finally, several tandemly arranged minirepeats of the form (TA), that are usually found at the 3' ends of the primate Alu family xKpynll family repeats, can be cleaved efficiently in a regular pattern by the enzyme. These data are discussed in terms of the mode of recognition of DNA seqeunces/structures by topoisomerase I, and its possible roles in the nonhomologous insertion of repetitive DNA sequences.
AB - The SDS-induced nicking of DNA helix by Hela topoisomerase I in vitro haa been studied by using 2.9 kb of cloned human DNA as the substrate. The frequency of nicking is increased from 1/23 (nick/nt) to 1/19 (nick/nt) when camptothecin is present in the nicking reaction. The cytotoxic drug also induces DNA nicks without the addition of SDS. Although the consensus built from DNA sequences from -20 to +20 of more tham one hundred of the nicking sites only shows a preference for T at position -1, the distributions of the topoisomerae I-cleavable sites among different categories of specific DNA sequences are apparently non- random. Long stretches of tandem (CA), A, or T residues, and the GC-rich promoter region of C globin gene are all refractory to the nicking reaction. Howevar, the nicking frequencies of short direct repeats flanking different Alu type sequences are as high as 1/6 (nick/nt). Finally, several tandemly arranged minirepeats of the form (TA), that are usually found at the 3' ends of the primate Alu family xKpynll family repeats, can be cleaved efficiently in a regular pattern by the enzyme. These data are discussed in terms of the mode of recognition of DNA seqeunces/structures by topoisomerase I, and its possible roles in the nonhomologous insertion of repetitive DNA sequences.
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U2 - 10.1093/nar/16.16.7975
DO - 10.1093/nar/16.16.7975
M3 - Article
C2 - 2843808
AN - SCOPUS:0023774273
SN - 0305-1048
VL - 16
SP - 7975
EP - 7993
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 16
ER -