Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates

Chia Hung Lee; Jun Lang; Chun Wan Yen; Pei Chun Shih; Tien Sung Lin; Chung Yuan Mou

doi:10.1021/jp050535k

Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates

Chia Hung Lee, Jun Lang, Chun Wan Yen, Pei Chun Shih, Tien Sung Lin, Chung Yuan Mou

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

The hydrothermal stability and oxidation activity of cytochrome c (cty c) by immobilization in the nanochannels of mesoporous aluminosilicates were discussed. The cytochrome c immobilized in MAS-9 undergo facile unfolding during hydrothermal stability. The stability and high catalytic activity of cyt c immobilized in mesoporous silicas were attributed to the electrostatic attraction between the pore surface and cyt c and the confinement provided by nanochannels. It was found that by increasing the concentration of the spin trapping agent, cyt c catalyzes a homolytic cleavage of the O-O bond of hydroperoxide and generates a protein cation radicals.

Original language	English
Pages (from-to)	12277-12286
Number of pages	10
Journal	Journal of Physical Chemistry B
Volume	109
Issue number	25
DOIs	https://doi.org/10.1021/jp050535k
Publication status	Published - Jun 30 2005
Externally published	Yes

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

Access to Document

10.1021/jp050535k

Cite this

@article{00f909e52a9847fb8ab8c3b685de05f3,

title = "Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates",

abstract = "The hydrothermal stability and oxidation activity of cytochrome c (cty c) by immobilization in the nanochannels of mesoporous aluminosilicates were discussed. The cytochrome c immobilized in MAS-9 undergo facile unfolding during hydrothermal stability. The stability and high catalytic activity of cyt c immobilized in mesoporous silicas were attributed to the electrostatic attraction between the pore surface and cyt c and the confinement provided by nanochannels. It was found that by increasing the concentration of the spin trapping agent, cyt c catalyzes a homolytic cleavage of the O-O bond of hydroperoxide and generates a protein cation radicals.",

author = "Lee, {Chia Hung} and Jun Lang and Yen, {Chun Wan} and Shih, {Pei Chun} and Lin, {Tien Sung} and Mou, {Chung Yuan}",

year = "2005",

month = jun,

day = "30",

doi = "10.1021/jp050535k",

language = "English",

volume = "109",

pages = "12277--12286",

journal = "Journal of Physical Chemistry B",

issn = "1520-6106",

number = "25",

}

TY - JOUR

T1 - Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates

AU - Lee, Chia Hung

AU - Lang, Jun

AU - Yen, Chun Wan

AU - Shih, Pei Chun

AU - Lin, Tien Sung

AU - Mou, Chung Yuan

PY - 2005/6/30

Y1 - 2005/6/30

N2 - The hydrothermal stability and oxidation activity of cytochrome c (cty c) by immobilization in the nanochannels of mesoporous aluminosilicates were discussed. The cytochrome c immobilized in MAS-9 undergo facile unfolding during hydrothermal stability. The stability and high catalytic activity of cyt c immobilized in mesoporous silicas were attributed to the electrostatic attraction between the pore surface and cyt c and the confinement provided by nanochannels. It was found that by increasing the concentration of the spin trapping agent, cyt c catalyzes a homolytic cleavage of the O-O bond of hydroperoxide and generates a protein cation radicals.

AB - The hydrothermal stability and oxidation activity of cytochrome c (cty c) by immobilization in the nanochannels of mesoporous aluminosilicates were discussed. The cytochrome c immobilized in MAS-9 undergo facile unfolding during hydrothermal stability. The stability and high catalytic activity of cyt c immobilized in mesoporous silicas were attributed to the electrostatic attraction between the pore surface and cyt c and the confinement provided by nanochannels. It was found that by increasing the concentration of the spin trapping agent, cyt c catalyzes a homolytic cleavage of the O-O bond of hydroperoxide and generates a protein cation radicals.

UR - http://www.scopus.com/inward/record.url?scp=22744457649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22744457649&partnerID=8YFLogxK

U2 - 10.1021/jp050535k

DO - 10.1021/jp050535k

M3 - Article

C2 - 16852515

AN - SCOPUS:22744457649

SN - 1520-6106

VL - 109

SP - 12277

EP - 12286

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

IS - 25

ER -

Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this