Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates

Chia Hung Lee, Jun Lang, Chun Wan Yen, Pei Chun Shih, Tien Sung Lin, Chung Yuan Mou

Research output: Contribution to journalArticlepeer-review

97 Citations (Scopus)

Abstract

The hydrothermal stability and oxidation activity of cytochrome c (cty c) by immobilization in the nanochannels of mesoporous aluminosilicates were discussed. The cytochrome c immobilized in MAS-9 undergo facile unfolding during hydrothermal stability. The stability and high catalytic activity of cyt c immobilized in mesoporous silicas were attributed to the electrostatic attraction between the pore surface and cyt c and the confinement provided by nanochannels. It was found that by increasing the concentration of the spin trapping agent, cyt c catalyzes a homolytic cleavage of the O-O bond of hydroperoxide and generates a protein cation radicals.

Original languageEnglish
Pages (from-to)12277-12286
Number of pages10
JournalJournal of Physical Chemistry B
Volume109
Issue number25
DOIs
Publication statusPublished - Jun 30 2005
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Enhancing stability and oxidation activity of cytochrome c by immobilization in the nanochannels of mesoporous aluminosilicates'. Together they form a unique fingerprint.

Cite this