Enhanced Specificity of Mint Geranyl Pyrophosphate Synthase by Modifying the R-Loop Interactions

Fu Lien Hsieh, Tao Hsin Chang, Tzu Ping Ko, Andrew H.J. Wang

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Isoprenoids, most of them synthesized by prenyltransferases (PTSs), are a class of important biologically active compounds with diverse functions. The mint geranyl pyrophosphate synthase (GPPS) is a heterotetramer composed of two LSU.SSU (large/small subunit) dimers. In addition to C10-GPP, the enzyme also produces geranylgeranyl pyrophosphate (C20-GGPP) in vitro, probably because of the conserved active-site structures between the LSU of mint GPPS and the homodimeric GGPP synthase from mustard. By contrast, the SSU lacks the conserved aspartate-rich motifs for catalysis. A major active-site cavity loop in the LSU and other trans-type PTSs is replaced by the regulatory R-loop in the SSU. Only C10-GPP, but not C20-GGPP, was produced when intersubunit interactions of the R-loop were disrupted by either deletion or multiple point mutations. The structure of the deletion mutant, determined in two different crystal forms, shows an intact (LSU.SSU)2 heterotetramer, as previously observed in the wild-type enzyme. The active-site of LSU remains largely unaltered, except being slightly more open to the bulk solvent. The R-loop of SSU acts by regulating the product release from LSU, just as does its equivalent loop in a homodimeric PTS, which prevents the early reaction intermediates from escaping the active site of the other subunit. In this way, the product-retaining function of R-loop provides a more stringent control for chain-length determination, complementary to the well-established molecular ruler mechanism. We conclude that the R-loop may be used not only to conserve the GPPS activity but also to produce portions of C20-GGPP in mint.

Original languageEnglish
Pages (from-to)859-873
Number of pages15
JournalJournal of Molecular Biology
Issue number5
Publication statusPublished - Dec 17 2010
Externally publishedYes


  • Heteromeric enzyme
  • Isoprenoid
  • Mentha piperita
  • Plant volatile
  • Prenyltransferase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology


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