Enhanced active extracellular polysaccharide production from Ganoderma formosanum using computational modeling

Kai Di Hsu, Shu Pei Wu, Shin Ping Lin, Chi Chin Lum, Kuan Chen Cheng

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Extracellular polysaccharide (EPS) is one of the major bioactive ingredients contributing to the health benefits of Ganoderma spp. In this study, response surface methodology was applied to determine the optimal culture conditions for EPS production of Ganoderma formosanum. The optimum medium composition was found to be at initial pH 5.3, 49.2 g/L of glucose, and 4.9 g/L of yeast extract by implementing a three-factor–three-level Box–Behnken design. Under this condition, the predicted yield of EPS was up to 830.2 mg/L, which was 1.4-fold higher than the one from basic medium (604.5 mg/L). Furthermore, validating the experimental value of EPS production depicted a high correlation (100.4%) with the computational prediction response model. In addition, the percentage of β-glucan, a well-recognized bioactive polysaccharide, in EPS was 53 ± 5.5%, which was higher than that from Ganoderma lucidum in a previous study. Moreover, results of monosaccharide composition analysis indicated that glucose was the major component of G. formosanum EPS, supporting a high β-glucan percentage in EPS. Taken together, this is the first study to investigate the influence of medium composition for G. formosanum EPS production as well as its β-glucan composition.

Original languageEnglish
Pages (from-to)804-811
Number of pages8
JournalJournal of Food and Drug Analysis
Issue number4
Publication statusPublished - Oct 1 2017
Externally publishedYes


  • extracellular polysaccharide
  • Ganoderma formosanum
  • medium composition
  • response surface methodology
  • β-glucan

ASJC Scopus subject areas

  • Food Science
  • Pharmacology


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