Engineering three-dimensional collagen-IKVAV matrix to mimic neural microenvironment

Hossein Hosseinkhani, Yosuke Hiraoka, Chung Hsing Li, Yi Ru Chen, Dah Shyong Yu, Po Da Hong, Keng Liang Ou

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)


Engineering the cellular microenvironment has great potential to create a platform technology toward engineering of tissue and organs. This study aims to engineer a neural microenvironment through fabrication of three-dimensional (3D) engineered collagen matrixes mimicking in-vivo-like conditions. Collagen was chemically modified with a pentapeptide epitope consisting of isoleucine-lysine-valine-alanine-valine (IKVAV) to mimic laminin structure supports of the neural extracellular matrix (ECM). Three-dimensional collagen matrixes with and without IKVAV peptide modification were fabricated by freeze-drying technology and chemical cross-linking with glutaraldehyde. Structural information of 3D collagen matrixes indicated interconnected pores structure with an average pore size of 180 μm. Our results indicated that culture of dorsal root ganglion (DRG) cells in 3D collagen matrix was greatly influenced by 3D culture method and significantly enhanced with engineered collagen matrix conjugated with IKVAV peptide. It may be concluded that an appropriate 3D culture of neurons enables DRG to positively improve the cellular fate toward further acceleration in tissue regeneration.

Original languageEnglish
Pages (from-to)1229-1235
Number of pages7
JournalACS Chemical Neuroscience
Issue number8
Publication statusPublished - Aug 21 2013


  • 3D matrix
  • Tissue engineering
  • collagen
  • peptide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology


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