TY - JOUR
T1 - Endotoxin contamination contributes to the in vitro cytokine-inducing activity of osteopontin preparations
AU - Konno, Satoshi
AU - Hoshi, Takuya
AU - Taira, Toshio
AU - Plunkett, Beverly
AU - Huang, Shau Ku
PY - 2005/5
Y1 - 2005/5
N2 - Recently, both native and recombinant preparations of human osteopontin (OPN) have been shown to be able to induce the production of several proinflammatory cytokines in human peripheral blood mononuclear cells (PBMCs) or purified monocytes. In the present study, we found that commercially available native and recombinant OPNs contain variable amounts of endotoxin (LPS) and that removal of endotoxin by polymyxin B-agarose column abrogated their cytokine-inducing activity. These results suggest the questionable evidence of the ability of OPN to induce several cytokines in human PBMCs and draw attention to the exquisite sensitivity of PBMCs/monocytes to endotoxin contaminants.
AB - Recently, both native and recombinant preparations of human osteopontin (OPN) have been shown to be able to induce the production of several proinflammatory cytokines in human peripheral blood mononuclear cells (PBMCs) or purified monocytes. In the present study, we found that commercially available native and recombinant OPNs contain variable amounts of endotoxin (LPS) and that removal of endotoxin by polymyxin B-agarose column abrogated their cytokine-inducing activity. These results suggest the questionable evidence of the ability of OPN to induce several cytokines in human PBMCs and draw attention to the exquisite sensitivity of PBMCs/monocytes to endotoxin contaminants.
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U2 - 10.1089/jir.2005.25.277
DO - 10.1089/jir.2005.25.277
M3 - Article
C2 - 15871665
AN - SCOPUS:18644385891
SN - 1079-9907
VL - 25
SP - 277
EP - 282
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 5
ER -