Abstract
A highly sensitive method for the determination of D-lactate in rat urine was developed by using a high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) connected to a chiral column. At first, (D+L)-lactate in the urine were derivatized with a fluorescent reagent, 4-nitro-7-piperazino-2,1, 3-benzoxadiazole (NBD-PZ), and separated on the ODS column and determined fluorimetrically at 547 nm with 491 nm of excitation wavelength. During the separation step on the ODS, the peak fraction of (D+L)-lactate derivative was introduced directly to an amylose tris (3,5-dimethylphenylcarbamate) (Chiralpak AD-RH) chiral column by changing the flow of the eluent via 6-port valve. Then, D-lactate derivative was separated enantiomerically from L-lactate derivative, and the enantiomeric ratio was determined from the chromatogram. The accuracy values for the determination of D-lactate in 20 μL of rat urine were 96.93% -104.85%. The intra- and inter-day precision values were within 0.80% and 14.44%. The proposed method was applied to the urine of diabetic rats induced by intraperitoneal administration of streptozotocin, and the significant increases of D-lactate was observed in the diabetic rats as compared to the normal rats.
Original language | English |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Editors | C. Quan, F.S. Chau, A. Asundi, B.S. Wong, C.T. Lim |
Pages | 954-958 |
Number of pages | 5 |
Volume | 5852 PART II |
DOIs | |
Publication status | Published - 2005 |
Event | Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics - Singapore, Singapore Duration: Nov 29 2004 → Dec 1 2004 |
Other
Other | Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics |
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Country/Territory | Singapore |
City | Singapore |
Period | 11/29/04 → 12/1/04 |
Keywords
- Chiralpak AD-RH
- D-lactate
- Enantiomeric determination
- NBD-PZ
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics