Elevated nitric oxide level in aqueous humor of AIDS patients with cytomegalovirus retinitis

Wen Ming Hsu; Steve S.L. Chen; Chi Hsien Peng; Chieh Fu Chen; Yu Chieh Ko; Der Chong Tsai; Ching Kuang Chou; Larry L.T. Ho; Shih Hwa Chiou; Jorn Hon Liu

doi:10.1159/000070639

Elevated nitric oxide level in aqueous humor of AIDS patients with cytomegalovirus retinitis

Wen Ming Hsu, Steve S.L. Chen, Chi Hsien Peng, Chieh Fu Chen, Yu Chieh Ko, Der Chong Tsai, Ching Kuang Chou, Larry L.T. Ho, Shih Hwa Chiou, Jorn Hon Liu

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) retinitis is the most common ocular opportunistic infection in AIDS. It often leads to blindness if left untreated. The questions as to how HCMV infection causes retinal immunopathogenesis and visual destruction in AIDS patients have not been completely established. Here we reported that the nitric oxide (NO) levels in aqueous humor samples in 10 AIDS patients with CMV retinitis (104.3 ± 27.1 μM) were higher than the levels in 7 AIDS patients without CMV retinitis (36.1 ± 10.4 μM; p < 0.001). After ganciclovir treatment, the NO level in the vitreous body of 5 patients declined dramatically (53.4 ± 11.8 μM). By using immunohistochemistry assay, we found that the aggregates of macrophages infiltrated in the CMV-infected retina of 4 AIDS patients. Moreover, the expression of inducible-form NO synthase was detected in the infected retina of these patients. These results suggest that NO production in the eye may play a fundamental role in the immunopathogenesis of AIDS patients with CMV retinitis.

Original language	English
Pages (from-to)	298-301
Number of pages	4
Journal	Ophthalmologica
Volume	217
Issue number	4
DOIs	https://doi.org/10.1159/000070639
Publication status	Published - 2003
Externally published	Yes

Keywords

AIDS
Cytomegalovirus
Macrophage
Nitric oxide

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000070639

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title = "Elevated nitric oxide level in aqueous humor of AIDS patients with cytomegalovirus retinitis",

abstract = "Human cytomegalovirus (HCMV) retinitis is the most common ocular opportunistic infection in AIDS. It often leads to blindness if left untreated. The questions as to how HCMV infection causes retinal immunopathogenesis and visual destruction in AIDS patients have not been completely established. Here we reported that the nitric oxide (NO) levels in aqueous humor samples in 10 AIDS patients with CMV retinitis (104.3 ± 27.1 μM) were higher than the levels in 7 AIDS patients without CMV retinitis (36.1 ± 10.4 μM; p < 0.001). After ganciclovir treatment, the NO level in the vitreous body of 5 patients declined dramatically (53.4 ± 11.8 μM). By using immunohistochemistry assay, we found that the aggregates of macrophages infiltrated in the CMV-infected retina of 4 AIDS patients. Moreover, the expression of inducible-form NO synthase was detected in the infected retina of these patients. These results suggest that NO production in the eye may play a fundamental role in the immunopathogenesis of AIDS patients with CMV retinitis.",

keywords = "AIDS, Cytomegalovirus, Macrophage, Nitric oxide",

author = "Hsu, {Wen Ming} and Chen, {Steve S.L.} and Peng, {Chi Hsien} and Chen, {Chieh Fu} and Ko, {Yu Chieh} and Tsai, {Der Chong} and Chou, {Ching Kuang} and Ho, {Larry L.T.} and Chiou, {Shih Hwa} and Liu, {Jorn Hon}",

year = "2003",

doi = "10.1159/000070639",

language = "English",

volume = "217",

pages = "298--301",

journal = "Ophthalmologica",

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TY - JOUR

T1 - Elevated nitric oxide level in aqueous humor of AIDS patients with cytomegalovirus retinitis

AU - Hsu, Wen Ming

AU - Chen, Steve S.L.

AU - Peng, Chi Hsien

AU - Chen, Chieh Fu

AU - Ko, Yu Chieh

AU - Tsai, Der Chong

AU - Chou, Ching Kuang

AU - Ho, Larry L.T.

AU - Chiou, Shih Hwa

AU - Liu, Jorn Hon

PY - 2003

Y1 - 2003

N2 - Human cytomegalovirus (HCMV) retinitis is the most common ocular opportunistic infection in AIDS. It often leads to blindness if left untreated. The questions as to how HCMV infection causes retinal immunopathogenesis and visual destruction in AIDS patients have not been completely established. Here we reported that the nitric oxide (NO) levels in aqueous humor samples in 10 AIDS patients with CMV retinitis (104.3 ± 27.1 μM) were higher than the levels in 7 AIDS patients without CMV retinitis (36.1 ± 10.4 μM; p < 0.001). After ganciclovir treatment, the NO level in the vitreous body of 5 patients declined dramatically (53.4 ± 11.8 μM). By using immunohistochemistry assay, we found that the aggregates of macrophages infiltrated in the CMV-infected retina of 4 AIDS patients. Moreover, the expression of inducible-form NO synthase was detected in the infected retina of these patients. These results suggest that NO production in the eye may play a fundamental role in the immunopathogenesis of AIDS patients with CMV retinitis.

AB - Human cytomegalovirus (HCMV) retinitis is the most common ocular opportunistic infection in AIDS. It often leads to blindness if left untreated. The questions as to how HCMV infection causes retinal immunopathogenesis and visual destruction in AIDS patients have not been completely established. Here we reported that the nitric oxide (NO) levels in aqueous humor samples in 10 AIDS patients with CMV retinitis (104.3 ± 27.1 μM) were higher than the levels in 7 AIDS patients without CMV retinitis (36.1 ± 10.4 μM; p < 0.001). After ganciclovir treatment, the NO level in the vitreous body of 5 patients declined dramatically (53.4 ± 11.8 μM). By using immunohistochemistry assay, we found that the aggregates of macrophages infiltrated in the CMV-infected retina of 4 AIDS patients. Moreover, the expression of inducible-form NO synthase was detected in the infected retina of these patients. These results suggest that NO production in the eye may play a fundamental role in the immunopathogenesis of AIDS patients with CMV retinitis.

KW - AIDS

KW - Cytomegalovirus

KW - Macrophage

KW - Nitric oxide

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ER -

Elevated nitric oxide level in aqueous humor of AIDS patients with cytomegalovirus retinitis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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