TY - JOUR
T1 - Electroporation for three commonly used yeast strains for two-hybrid screening experiments
AU - Lin, Hsin Ying
AU - Lin, Sey En
AU - Chien, Su Fang
AU - Chern, Ming Kai
PY - 2011/9/1
Y1 - 2011/9/1
N2 - The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
AB - The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
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U2 - 10.1016/j.ab.2011.04.038
DO - 10.1016/j.ab.2011.04.038
M3 - Article
C2 - 21601557
AN - SCOPUS:79959768607
SN - 0003-2697
VL - 416
SP - 117
EP - 119
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -