TY - JOUR
T1 - EGF-R as a hemopoietic growth factor receptor
T2 - The c-erbB product is present in chicken erythrocytic progenitors and controls their self-renewal
AU - Pain, B.
AU - Woods, C. M.
AU - Saez, J.
AU - Flickinger, T.
AU - Raines, M.
AU - Peyroll, S.
AU - Moscovici, C.
AU - Moscovici, M. G.
AU - Kung, H. J.
AU - Jurdic, P.
AU - Lazarides, E.
AU - Samarut, J.
N1 - Funding Information:
We are grateful to M. A. Maillard for typing the manuscript. This work was supported by grants to J. S. from CNRS, INSERM, INRA, ARC, and the F~d6ration des Centres de Lutte Contre le Cancer; to E. L. from the American Cancer Society, the National Science Foundation, and the Lucille P. Markey Charitable Trust to the Division of Biology, California Institute of Technology; and to H.-J. K. from the National Cancer Institute (CA39207) and from Edison Biotschnology Center of Ohio. During the tenure of this work E. L. held the visiting Chair Marcel M(~rieux at ENS de Lyon supported by the Fondation M6rieux.
PY - 1991/4/5
Y1 - 1991/4/5
N2 - c-erbB, encoding the EGF receptor (EGF-R), was originally identified as the cellular homolog of a chicken leukemia oncogene. In humans, EGF-R is distributed widely except in hemopoietic tissues, and its amplification is associated with epidermal and glial malignancies. Here we show that c-erbB is present in normal chicken erythrocytic progenitors and transmits the mitogenic signal induced by TGFα. Cells that contain high affinity EGF-R are at approximately the BFU-E stage, and their long-term renewal can be Induced by TGFa. Upon addition of insulin and erythropoletin, they can be induced to terminally differentiate into red cells. We previously demonstrated that v-erbA blocks differentiation of chicken erythrocytic progenitors but does not abrogate their growth factor dependence for proliferation. These data indicate that proliferation and differentiation are not necessarily coupled in these cells. They also demonstrate a direct role of c-erbB in the control of self-renewal of normal chicken erythrocytic progenitors and could account for the predominant leukemogenic potential of the chicken erbB gene.
AB - c-erbB, encoding the EGF receptor (EGF-R), was originally identified as the cellular homolog of a chicken leukemia oncogene. In humans, EGF-R is distributed widely except in hemopoietic tissues, and its amplification is associated with epidermal and glial malignancies. Here we show that c-erbB is present in normal chicken erythrocytic progenitors and transmits the mitogenic signal induced by TGFα. Cells that contain high affinity EGF-R are at approximately the BFU-E stage, and their long-term renewal can be Induced by TGFa. Upon addition of insulin and erythropoletin, they can be induced to terminally differentiate into red cells. We previously demonstrated that v-erbA blocks differentiation of chicken erythrocytic progenitors but does not abrogate their growth factor dependence for proliferation. These data indicate that proliferation and differentiation are not necessarily coupled in these cells. They also demonstrate a direct role of c-erbB in the control of self-renewal of normal chicken erythrocytic progenitors and could account for the predominant leukemogenic potential of the chicken erbB gene.
UR - http://www.scopus.com/inward/record.url?scp=0025752493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025752493&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(91)90405-N
DO - 10.1016/0092-8674(91)90405-N
M3 - Article
C2 - 1672832
AN - SCOPUS:0025752493
SN - 0092-8674
VL - 65
SP - 37
EP - 46
JO - Cell
JF - Cell
IS - 1
ER -