Effects of the nanostructure and nanoporosity on bioactive nanohydroxyapatite/reconstituted collagen by electrodeposition

Keng Liang Ou, Jeffery Wu, Wen-FuThomas Lai, Charng Bin Yang, Wen Chang Lo, Li Hsuan Chiu, John Bowley

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


Hydroxyapatite (HA)/collagen composites were reported to induce bony growth. Various methods for preparing HA-based composites have been investigated as potential biomaterials for bone substitutes. However, no method can generate a thick nanoporous HA. A novel bone regenerative nanocomposite consisting of nanohydroxyapatite (HA), nano-amorphous calcium phosphate (ACP) and reconstituted collagen by electrodeposition was designed in this research. Specimens with and without nanoporosity were evaluated using electrochemical measurements, material analyses, and cell-material interactions. The results showed that reconstituted collagen/nano-(HA and ACP) illustrated a multinanoporous structure and enhanced biocompatibility. Nanocomposite was comprised to nano-(HA and ACP) and reconstituted collagen. The core cell structure was formed during electrodeposition. Nanoporosity and nanostructure were observed as formation of nanocomposite. The nano-(HA and ACP) phases were essentially composed of a nanoporous and nanostructural biocomposite. Reconstituted collagen incorporation with the nanoporous and nanostructural biocomposite significantly facilitated the osteogenic differentiation of mesenchymal stem cells. Reconstituted collagen was covered with nano-(HA and ACP), profoundly impacting the enhancement of biocompatibility on application of implant and tissue engineering. The bioactive nano-HA/reconstituted collagen-induced osteogenic differentiation of mesenchymal stem cells enables to enhance bone growth/ repair and osseointegration.

Original languageEnglish
Pages (from-to)906-912
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Issue number3
Publication statusPublished - Mar 1 2010


  • Electrodeposition
  • Mesenchymal stem cell
  • Nano-amorphous calcium phosphate
  • Nano-hydroxyapatite
  • Reconstituted collagen

ASJC Scopus subject areas

  • Ceramics and Composites
  • Metals and Alloys
  • Biomedical Engineering
  • Biomaterials


Dive into the research topics of 'Effects of the nanostructure and nanoporosity on bioactive nanohydroxyapatite/reconstituted collagen by electrodeposition'. Together they form a unique fingerprint.

Cite this