Effects of prophylactic administration of glutamine on CD4 + T cell polarisation and kidney injury in mice with polymicrobial sepsis

Yu Chen Hou, Jin Ming Wu, Kuen Yuan Chen, Po Da Chen, Cing Syuan Lei, Sung Ling Yeh, Ming Tsan Lin

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14 Citations (Scopus)


The present study investigated the effects of glutamine (GLN) pretreatment on CD4 + T cell polarisation and remote kidney injury in mice with gut-derived polymicrobial sepsis. Mice were randomly assigned to three groups: normal control fed with American Institute of Nutrition (AIN)-93G diet and two sepsis groups provided with either AIN-93G-based diet or identical components, except part of casein was replaced by GLN. Mice were given their respective diets for 2 weeks. Then, mice in the sepsis groups were performed with caecal ligation and puncture and were killed 72 h after the surgery. Blood, spleens and kidneys were collected for further examination. The results showed that sepsis resulted in decreased circulating and splenic total T lymphocyte and CD4 + T cell percentages, whereas IL-4-, and forkhead box p3 (Foxp3)-expressing CD4 + T cells percentages were up-regulated. Compared with the sepsis control group, pretreatment with GLN maintained blood T and CD4 + T cells and reduced percentages of IL-4- and Foxp3-expressing CD4 + T cells. Also, a more pronounced activation and increased anti-apoptotic Bcl-2 gene expression of splenic CD4 + T cells were observed. Concomitant with the decreased plasma IL-6, keratinocyte-derived chemokine (KC) levels, the gene expression of KC, macrophage inflammatory protein-2 and renal injury biomarker kidney injury molecule-1 (Kim-1) were down-regulated when GLN was administered. These findings suggest that antecedent of GLN administration elicit a more balanced blood T helper cell polarisation, sustained T cell populations, prevented splenic CD4 + T cell apoptosis and attenuated kidney injury at late phase of polymicrobial sepsis. GLN may have benefits in subjects at risk of abdominal infection.

Original languageEnglish
Pages (from-to)657-665
Number of pages9
JournalBritish Journal of Nutrition
Issue number6
Publication statusPublished - Sept 28 2019


  • CD4 T cells
  • Glutamine
  • Kidney injury
  • Sepsis
  • T cell apoptosis
  • CD4+ T cells

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Medicine (miscellaneous)


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