Effects of plant- and animal-based high-fat diets on lipid storage and distribution in environmental bacteria-colonized gnotobiotic mice

Yi Hsun Chen, Chien Chao Chiu, Shao Wen Hung, Ju Yun Liu, Yu Chih Wang, Qiang Lv, Cheng Chih Hsu, Yi Wen Huang, Wen Ching Huang, Hsiao Li Chuang, Ter Hsin Chen

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Different edible oils such as lard and soybean oil have been reported to interact with the gut microbiota, affecting host lipid metabolism. However, whether bacteria derived from the environment influence host lipid metabolism remains unclear. This study aimed to clarify the roles of environmental bacteria in host lipid storage and distribution with various edible oils. Gnotobiotic C57BL/6JNarl mice were inoculated with Lysinibacillus xylanilyticus and Paenibacillus azoreducens and then fed either a normal diet (LabDiet 5010, control group) or a diet containing 60% lard (L-group) or soybean oil (S-group) for 18 months. Interestingly, the S-group accumulated massive amounts of white adipose tissue compared to the L- and control groups, while the L-group displayed more hepatic steatosis and fatty droplets than the other groups. The expression of fatty acid synthase (FAS), hydroxymethylglutaryl-coenzyme A reductase (HMGCR), sterol regulatory element-binding protein 2 (SREBP2), and peroxisome proliferator-activated receptor gamma (PPARγ) in the livers of the L-group were markedly elevated compared to the S-group. FAS and PPARγ protein levels were also markedly elevated. However, there were no differences in the expression of the pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-α between the groups. Our results suggest that environmental bacteria may affect host hepatic inflammation and lipid distribution in the presence of high-fat diets, with different effects depending on the fat type consumed.

Original languageEnglish
Pages (from-to)1075-1081
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume493
Issue number2
DOIs
Publication statusPublished - Nov 18 2017

Keywords

  • Environmental bacteria
  • Gnotobiotic mouse
  • Hepatic steatosis
  • Inflammation
  • Lard
  • Soybean oil

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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