Effects of parenteral structured lipid emulsion on modulating the inflammatory response in rats undergoing a total gastrectomy

Ming Tsan Lin; Sung Ling Yeh; Shung Sheng Tsou; Ming Yang Wang; Wei Jao Chen

doi:10.1016/j.nut.2008.07.015

Effects of parenteral structured lipid emulsion on modulating the inflammatory response in rats undergoing a total gastrectomy

Ming Tsan Lin, Sung Ling Yeh, Shung Sheng Tsou, Ming Yang Wang, Wei Jao Chen

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Objectives: Structured lipid emulsion improves the nitrogen balance and is rapidly cleared from the blood of moderately catabolic patients. However, the effects of structured lipids on inflammatory reactions during major surgery are not clear. This study investigated the effect of a parenteral structured triacylglycerol emulsion on leukocyte adhesion molecule expression and inflammatory mediator production in rats undergoing a total gastrectomy. Methods: Normal rats with internal jugular catheters were assigned to three experimental groups and received total parenteral nutrition. At the same time, a total gastrectomy was performed on the experimental groups. The total parenteral nutrition solutions were isonitrogenous and identical in nutrient compositions except for differences in the composition of the fat emulsion. Group 1 received a conventional fat emulsion with long-chain triacylglycerols (LCTs), group 2 received a physical mixture of medium-chain triacylglycerols (MCTs) and LCTs (MCT/LCT), and group 3 received structured lipids composed of MCTs and LCTs (STG). Half of the rats in each respective group were sacrificed 1 d and the other half 3 d after surgery to examine the analytical parameters. Results: Plasma cholesterol and free fatty acid levels in the STG group were lower than those in the other groups after surgery. The STG group had lower leukocyte CD11a/CD18 expressions than the MCT/LCT group 3 d after surgery, and CD11b/CD18 expressions in the STG group were lower than those in the LCT group on postoperative days. The STG group had higher monocyte chemotactic protein-1 and macrophage inflammatory protein-2 levels in peritoneal lavage fluid than did the other two groups. Conclusion: These results suggest that, compared with the LCT and MCT/LCT groups, rats administered STG had lower plasma lipid concentrations and leukocyte integrin expressions. In addition, STG administration may cause increased recruiting of neutrophils and monocytes at the site of injury and enhance antipathogenicity in rats undergoing a total gastrectomy.

Original language	English
Pages (from-to)	115-121
Number of pages	7
Journal	Nutrition
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/j.nut.2008.07.015
Publication status	Published - Jan 2009

Keywords

Adhesion molecule
Gastrectomy
Inflammatory mediator
Long-chain triacylglycerol
Medium-chain triacylglycerol
Structured lipids

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.nut.2008.07.015

Cite this

@article{2a957979c5ce4652af760cab3606661b,

title = "Effects of parenteral structured lipid emulsion on modulating the inflammatory response in rats undergoing a total gastrectomy",

abstract = "Objectives: Structured lipid emulsion improves the nitrogen balance and is rapidly cleared from the blood of moderately catabolic patients. However, the effects of structured lipids on inflammatory reactions during major surgery are not clear. This study investigated the effect of a parenteral structured triacylglycerol emulsion on leukocyte adhesion molecule expression and inflammatory mediator production in rats undergoing a total gastrectomy. Methods: Normal rats with internal jugular catheters were assigned to three experimental groups and received total parenteral nutrition. At the same time, a total gastrectomy was performed on the experimental groups. The total parenteral nutrition solutions were isonitrogenous and identical in nutrient compositions except for differences in the composition of the fat emulsion. Group 1 received a conventional fat emulsion with long-chain triacylglycerols (LCTs), group 2 received a physical mixture of medium-chain triacylglycerols (MCTs) and LCTs (MCT/LCT), and group 3 received structured lipids composed of MCTs and LCTs (STG). Half of the rats in each respective group were sacrificed 1 d and the other half 3 d after surgery to examine the analytical parameters. Results: Plasma cholesterol and free fatty acid levels in the STG group were lower than those in the other groups after surgery. The STG group had lower leukocyte CD11a/CD18 expressions than the MCT/LCT group 3 d after surgery, and CD11b/CD18 expressions in the STG group were lower than those in the LCT group on postoperative days. The STG group had higher monocyte chemotactic protein-1 and macrophage inflammatory protein-2 levels in peritoneal lavage fluid than did the other two groups. Conclusion: These results suggest that, compared with the LCT and MCT/LCT groups, rats administered STG had lower plasma lipid concentrations and leukocyte integrin expressions. In addition, STG administration may cause increased recruiting of neutrophils and monocytes at the site of injury and enhance antipathogenicity in rats undergoing a total gastrectomy.",

keywords = "Adhesion molecule, Gastrectomy, Inflammatory mediator, Long-chain triacylglycerol, Medium-chain triacylglycerol, Structured lipids",

author = "Lin, {Ming Tsan} and Yeh, {Sung Ling} and Tsou, {Shung Sheng} and Wang, {Ming Yang} and Chen, {Wei Jao}",

note = "Funding Information: This study was supported by research grant NSC95-2314-B002-156 from the National Science Council, Republic of China. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

month = jan,

doi = "10.1016/j.nut.2008.07.015",

language = "English",

volume = "25",

pages = "115--121",

journal = "Nutrition",

issn = "0899-9007",

publisher = "Elsevier Inc.",

number = "1",

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TY - JOUR

T1 - Effects of parenteral structured lipid emulsion on modulating the inflammatory response in rats undergoing a total gastrectomy

AU - Lin, Ming Tsan

AU - Yeh, Sung Ling

AU - Tsou, Shung Sheng

AU - Wang, Ming Yang

AU - Chen, Wei Jao

N1 - Funding Information: This study was supported by research grant NSC95-2314-B002-156 from the National Science Council, Republic of China. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/1

Y1 - 2009/1

N2 - Objectives: Structured lipid emulsion improves the nitrogen balance and is rapidly cleared from the blood of moderately catabolic patients. However, the effects of structured lipids on inflammatory reactions during major surgery are not clear. This study investigated the effect of a parenteral structured triacylglycerol emulsion on leukocyte adhesion molecule expression and inflammatory mediator production in rats undergoing a total gastrectomy. Methods: Normal rats with internal jugular catheters were assigned to three experimental groups and received total parenteral nutrition. At the same time, a total gastrectomy was performed on the experimental groups. The total parenteral nutrition solutions were isonitrogenous and identical in nutrient compositions except for differences in the composition of the fat emulsion. Group 1 received a conventional fat emulsion with long-chain triacylglycerols (LCTs), group 2 received a physical mixture of medium-chain triacylglycerols (MCTs) and LCTs (MCT/LCT), and group 3 received structured lipids composed of MCTs and LCTs (STG). Half of the rats in each respective group were sacrificed 1 d and the other half 3 d after surgery to examine the analytical parameters. Results: Plasma cholesterol and free fatty acid levels in the STG group were lower than those in the other groups after surgery. The STG group had lower leukocyte CD11a/CD18 expressions than the MCT/LCT group 3 d after surgery, and CD11b/CD18 expressions in the STG group were lower than those in the LCT group on postoperative days. The STG group had higher monocyte chemotactic protein-1 and macrophage inflammatory protein-2 levels in peritoneal lavage fluid than did the other two groups. Conclusion: These results suggest that, compared with the LCT and MCT/LCT groups, rats administered STG had lower plasma lipid concentrations and leukocyte integrin expressions. In addition, STG administration may cause increased recruiting of neutrophils and monocytes at the site of injury and enhance antipathogenicity in rats undergoing a total gastrectomy.

AB - Objectives: Structured lipid emulsion improves the nitrogen balance and is rapidly cleared from the blood of moderately catabolic patients. However, the effects of structured lipids on inflammatory reactions during major surgery are not clear. This study investigated the effect of a parenteral structured triacylglycerol emulsion on leukocyte adhesion molecule expression and inflammatory mediator production in rats undergoing a total gastrectomy. Methods: Normal rats with internal jugular catheters were assigned to three experimental groups and received total parenteral nutrition. At the same time, a total gastrectomy was performed on the experimental groups. The total parenteral nutrition solutions were isonitrogenous and identical in nutrient compositions except for differences in the composition of the fat emulsion. Group 1 received a conventional fat emulsion with long-chain triacylglycerols (LCTs), group 2 received a physical mixture of medium-chain triacylglycerols (MCTs) and LCTs (MCT/LCT), and group 3 received structured lipids composed of MCTs and LCTs (STG). Half of the rats in each respective group were sacrificed 1 d and the other half 3 d after surgery to examine the analytical parameters. Results: Plasma cholesterol and free fatty acid levels in the STG group were lower than those in the other groups after surgery. The STG group had lower leukocyte CD11a/CD18 expressions than the MCT/LCT group 3 d after surgery, and CD11b/CD18 expressions in the STG group were lower than those in the LCT group on postoperative days. The STG group had higher monocyte chemotactic protein-1 and macrophage inflammatory protein-2 levels in peritoneal lavage fluid than did the other two groups. Conclusion: These results suggest that, compared with the LCT and MCT/LCT groups, rats administered STG had lower plasma lipid concentrations and leukocyte integrin expressions. In addition, STG administration may cause increased recruiting of neutrophils and monocytes at the site of injury and enhance antipathogenicity in rats undergoing a total gastrectomy.

KW - Adhesion molecule

KW - Gastrectomy

KW - Inflammatory mediator

KW - Long-chain triacylglycerol

KW - Medium-chain triacylglycerol

KW - Structured lipids

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Effects of parenteral structured lipid emulsion on modulating the inflammatory response in rats undergoing a total gastrectomy

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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