Effects of non-protein-type amino acids of fine particulate matter on E-cadherin and inflammatory responses in mice

Hsiao Chi Chuang; Kin Fai Ho; Jun Ji Cao; Kai Jen Chuang; Steven Sai Hang Ho; Po Hao Feng; Linwei Tian; Chii Hong Lee; Yong Ming Han; Chun-Nin Lee; Tsun Jen Cheng

doi:10.1016/j.toxlet.2015.06.013

Effects of non-protein-type amino acids of fine particulate matter on E-cadherin and inflammatory responses in mice

Hsiao Chi Chuang, Kin Fai Ho, Jun Ji Cao, Kai Jen Chuang, Steven Sai Hang Ho, Po Hao Feng, Linwei Tian, Chii Hong Lee, Yong Ming Han, Chun-Nin Lee, Tsun Jen Cheng

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Exposure to particulate matter less than 2.5μm (PM_2.5) in size is an urgent issue for the protection of human health. Chemicals with PM_2.5 collected during a period of intensive haze episodes in Beijing (BJ), Xian (XA) and Hong Kong (HK) were characterised for organic carbon (OC), elemental carbon (EC), total carbon (TC) and free amino acids. BALB/c mice underwent aspiration exposure of 50 or 150μg of PM_2.5/mouse (BJ, XA and HK) on days 1 and 7 and were euthanised on day 14. The effects of these exposures on E-cadherin and inflammatory responses in the mouse lungs were analysed. The PM_2.5 chemicals consisted of significant amounts of OC: 36.6±17.2μg/m³ for BJ, 38.8±3.8μg/m³ for XA and 7.2±1.4μg/m³ for HK. A total of 23 free amino compounds for the PM_2.5 samples were analysed: 4075±1578pmol/m³ for BJ, 4718±2190pmol/m³ for XA and 1145±213pmol/m³ for HK. Exposure to PM_2.5 resulted in the suppression of E-cadherin levels in the lung tissues and increased IFN-γ, IL-2, IL-4, IL-6 and IL-10 in the bronchoalveolar lavage fluid. The alterations in E-cadherin, IFN-γ, IL-6 and IL-10 were associated with OC, TC and some amino acids, particularly non-protein-type amino acids. These data emphasised the deleterious health effects of PM_2.5.

Original language	English
Pages (from-to)	174-180
Number of pages	7
Journal	Toxicology Letters
Volume	237
Issue number	3
DOIs	https://doi.org/10.1016/j.toxlet.2015.06.013
Publication status	Published - Sept 7 2015

Keywords

Air pollution
Haze
Organic carbon
Oxidative stress
Physicochemistry
PM

ASJC Scopus subject areas

Toxicology
Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.toxlet.2015.06.013

Cite this

@article{b4fcbb3cebb7494b98819f1901c326a6,

title = "Effects of non-protein-type amino acids of fine particulate matter on E-cadherin and inflammatory responses in mice",

abstract = "Exposure to particulate matter less than 2.5μm (PM2.5) in size is an urgent issue for the protection of human health. Chemicals with PM2.5 collected during a period of intensive haze episodes in Beijing (BJ), Xian (XA) and Hong Kong (HK) were characterised for organic carbon (OC), elemental carbon (EC), total carbon (TC) and free amino acids. BALB/c mice underwent aspiration exposure of 50 or 150μg of PM2.5/mouse (BJ, XA and HK) on days 1 and 7 and were euthanised on day 14. The effects of these exposures on E-cadherin and inflammatory responses in the mouse lungs were analysed. The PM2.5 chemicals consisted of significant amounts of OC: 36.6±17.2μg/m3 for BJ, 38.8±3.8μg/m3 for XA and 7.2±1.4μg/m3 for HK. A total of 23 free amino compounds for the PM2.5 samples were analysed: 4075±1578pmol/m3 for BJ, 4718±2190pmol/m3 for XA and 1145±213pmol/m3 for HK. Exposure to PM2.5 resulted in the suppression of E-cadherin levels in the lung tissues and increased IFN-γ, IL-2, IL-4, IL-6 and IL-10 in the bronchoalveolar lavage fluid. The alterations in E-cadherin, IFN-γ, IL-6 and IL-10 were associated with OC, TC and some amino acids, particularly non-protein-type amino acids. These data emphasised the deleterious health effects of PM2.5.",

keywords = "Air pollution, Haze, Organic carbon, Oxidative stress, Physicochemistry, PM",

author = "Chuang, {Hsiao Chi} and Ho, {Kin Fai} and Cao, {Jun Ji} and Chuang, {Kai Jen} and Ho, {Steven Sai Hang} and Feng, {Po Hao} and Linwei Tian and Lee, {Chii Hong} and Han, {Yong Ming} and Chun-Nin Lee and Cheng, {Tsun Jen}",

year = "2015",

month = sep,

day = "7",

doi = "10.1016/j.toxlet.2015.06.013",

language = "English",

volume = "237",

pages = "174--180",

journal = "Toxicology Letters",

issn = "0378-4274",

publisher = "Elsevier BV",

number = "3",

}

TY - JOUR

T1 - Effects of non-protein-type amino acids of fine particulate matter on E-cadherin and inflammatory responses in mice

AU - Chuang, Hsiao Chi

AU - Ho, Kin Fai

AU - Cao, Jun Ji

AU - Chuang, Kai Jen

AU - Ho, Steven Sai Hang

AU - Feng, Po Hao

AU - Tian, Linwei

AU - Lee, Chii Hong

AU - Han, Yong Ming

AU - Lee, Chun-Nin

AU - Cheng, Tsun Jen

PY - 2015/9/7

Y1 - 2015/9/7

N2 - Exposure to particulate matter less than 2.5μm (PM2.5) in size is an urgent issue for the protection of human health. Chemicals with PM2.5 collected during a period of intensive haze episodes in Beijing (BJ), Xian (XA) and Hong Kong (HK) were characterised for organic carbon (OC), elemental carbon (EC), total carbon (TC) and free amino acids. BALB/c mice underwent aspiration exposure of 50 or 150μg of PM2.5/mouse (BJ, XA and HK) on days 1 and 7 and were euthanised on day 14. The effects of these exposures on E-cadherin and inflammatory responses in the mouse lungs were analysed. The PM2.5 chemicals consisted of significant amounts of OC: 36.6±17.2μg/m3 for BJ, 38.8±3.8μg/m3 for XA and 7.2±1.4μg/m3 for HK. A total of 23 free amino compounds for the PM2.5 samples were analysed: 4075±1578pmol/m3 for BJ, 4718±2190pmol/m3 for XA and 1145±213pmol/m3 for HK. Exposure to PM2.5 resulted in the suppression of E-cadherin levels in the lung tissues and increased IFN-γ, IL-2, IL-4, IL-6 and IL-10 in the bronchoalveolar lavage fluid. The alterations in E-cadherin, IFN-γ, IL-6 and IL-10 were associated with OC, TC and some amino acids, particularly non-protein-type amino acids. These data emphasised the deleterious health effects of PM2.5.

AB - Exposure to particulate matter less than 2.5μm (PM2.5) in size is an urgent issue for the protection of human health. Chemicals with PM2.5 collected during a period of intensive haze episodes in Beijing (BJ), Xian (XA) and Hong Kong (HK) were characterised for organic carbon (OC), elemental carbon (EC), total carbon (TC) and free amino acids. BALB/c mice underwent aspiration exposure of 50 or 150μg of PM2.5/mouse (BJ, XA and HK) on days 1 and 7 and were euthanised on day 14. The effects of these exposures on E-cadherin and inflammatory responses in the mouse lungs were analysed. The PM2.5 chemicals consisted of significant amounts of OC: 36.6±17.2μg/m3 for BJ, 38.8±3.8μg/m3 for XA and 7.2±1.4μg/m3 for HK. A total of 23 free amino compounds for the PM2.5 samples were analysed: 4075±1578pmol/m3 for BJ, 4718±2190pmol/m3 for XA and 1145±213pmol/m3 for HK. Exposure to PM2.5 resulted in the suppression of E-cadherin levels in the lung tissues and increased IFN-γ, IL-2, IL-4, IL-6 and IL-10 in the bronchoalveolar lavage fluid. The alterations in E-cadherin, IFN-γ, IL-6 and IL-10 were associated with OC, TC and some amino acids, particularly non-protein-type amino acids. These data emphasised the deleterious health effects of PM2.5.

KW - Air pollution

KW - Haze

KW - Organic carbon

KW - Oxidative stress

KW - Physicochemistry

KW - PM

UR - http://www.scopus.com/inward/record.url?scp=84934916673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934916673&partnerID=8YFLogxK

U2 - 10.1016/j.toxlet.2015.06.013

DO - 10.1016/j.toxlet.2015.06.013

M3 - Article

C2 - 26101798

AN - SCOPUS:84934916673

SN - 0378-4274

VL - 237

SP - 174

EP - 180

JO - Toxicology Letters

JF - Toxicology Letters

IS - 3

ER -

Effects of non-protein-type amino acids of fine particulate matter on E-cadherin and inflammatory responses in mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this