Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice

Ya Min Chang; Chen Tien Chang; Tzu Chuan Huang; Shih Ming Chen; Jen Ai Lee; Yun Chin Chung

doi:10.1016/j.foodchem.2011.06.044

Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice

Ya Min Chang, Chen Tien Chang, Tzu Chuan Huang, Shih Ming Chen, Jen Ai Lee, Yun Chin Chung

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.

Original language	English
Pages (from-to)	1751-1758
Number of pages	8
Journal	Food Chemistry
Volume	129
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2011.06.044
Publication status	Published - Dec 15 2011

Keywords

Aristolochic acid
Bamboo chitosanase
Low molecular weight chitosans
Renal histopathology
Renal lesions

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2011.06.044

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title = "Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice",

abstract = "This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.",

keywords = "Aristolochic acid, Bamboo chitosanase, Low molecular weight chitosans, Renal histopathology, Renal lesions",

author = "Chang, {Ya Min} and Chang, {Chen Tien} and Huang, {Tzu Chuan} and Chen, {Shih Ming} and Lee, {Jen Ai} and Chung, {Yun Chin}",

note = "Funding Information: The National Science Council, Taiwan ( NSC98-2313-B-126-003-MY3 and NSC97-2313-B-126-003-MY3 ) partially supported this research. Its financial support is greatly appreciated. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2011",

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doi = "10.1016/j.foodchem.2011.06.044",

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AU - Chang, Chen Tien

AU - Huang, Tzu Chuan

AU - Chen, Shih Ming

AU - Lee, Jen Ai

AU - Chung, Yun Chin

N1 - Funding Information: The National Science Council, Taiwan ( NSC98-2313-B-126-003-MY3 and NSC97-2313-B-126-003-MY3 ) partially supported this research. Its financial support is greatly appreciated. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2011/12/15

Y1 - 2011/12/15

N2 - This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.

AB - This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.

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Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice

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