TY - JOUR
T1 - Effects of low-intensity pulsed ultrasound, dexamethasone/TGF-β1 and/or BMP-2 on the transcriptional expression of genes in human mesenchymal stem cells
T2 - Chondrogenic vs. osteogenic differentiation
AU - Lai, Chien Hung
AU - Chen, Shih Ching
AU - Chiu, Li Hsuan
AU - Yang, Charng Bin
AU - Tsai, Yu Hui
AU - Zuo, Chun S.
AU - Chang, Walter Hong Shong
AU - Lai, Wen-FuThomas
PY - 2010/6
Y1 - 2010/6
N2 - The effects of low-intensity pulsed ultrasound (LIPUS) on the differentiation of human mesenchymal stem cells (hMSCs) were investigated in this study. hMSCs were subjected to LIPUS with or without dexamethasone/transforming growth factor-β1 (TD) or bone morphogenetic protein-2 (BMP-2) and the effects of this treatment were assessed. TD-treated hMSCs exhibited characteristic chondrogenic morphology and increased messenger RNA (mRNA) expression of chondrogenic markers and LIPUS enhanced the chondrogenic differentiation of hMSCs treated with TD. The expression of Runx2, an osteogenic transcription factor was not altered in either TD treatment group; however, a significant increase was detected in the LIPUS only group. The osteogenic appearance exhibited 3 days after LIPUS and/or BMP-2 treatment. Increases in the mRNA expression levels of osteogenic markers, Runx2 and ALP were also detected. There was no additive or altered effect with combined LIPUS and BMP-2 treatment. LIPUS alone can increase osteogenic differentiation of hMSCs and LIPUS enhances TD-mediated chondrogenic differentiation of hMSCs. Clinically, LIPUS may differentially influence bone vs. cartilage repair.
AB - The effects of low-intensity pulsed ultrasound (LIPUS) on the differentiation of human mesenchymal stem cells (hMSCs) were investigated in this study. hMSCs were subjected to LIPUS with or without dexamethasone/transforming growth factor-β1 (TD) or bone morphogenetic protein-2 (BMP-2) and the effects of this treatment were assessed. TD-treated hMSCs exhibited characteristic chondrogenic morphology and increased messenger RNA (mRNA) expression of chondrogenic markers and LIPUS enhanced the chondrogenic differentiation of hMSCs treated with TD. The expression of Runx2, an osteogenic transcription factor was not altered in either TD treatment group; however, a significant increase was detected in the LIPUS only group. The osteogenic appearance exhibited 3 days after LIPUS and/or BMP-2 treatment. Increases in the mRNA expression levels of osteogenic markers, Runx2 and ALP were also detected. There was no additive or altered effect with combined LIPUS and BMP-2 treatment. LIPUS alone can increase osteogenic differentiation of hMSCs and LIPUS enhances TD-mediated chondrogenic differentiation of hMSCs. Clinically, LIPUS may differentially influence bone vs. cartilage repair.
KW - Chondrogenic differentiation
KW - Human mesenchymal stem cells
KW - Low-intensity pulsed ultrasound
KW - Osteogenic differentiation
KW - Transforming growth factor
UR - http://www.scopus.com/inward/record.url?scp=77953179643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953179643&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2010.03.014
DO - 10.1016/j.ultrasmedbio.2010.03.014
M3 - Article
C2 - 20510190
AN - SCOPUS:77953179643
SN - 0301-5629
VL - 36
SP - 1022
EP - 1033
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 6
ER -
