Effects of heat treatment of selective laser melting printed ti-6al-4v specimens on surface texture parameters and cell attachment

Pei Wen Peng, Jen Chang Yang, Wei Fang Lee, Chih Yuan Fang, Chun Ming Chang, I. Jan Chen, Chengpo Hsu, Tzu Sen Yang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Selective laser melting (SLM) is extensively used for fabricating metallic biomedical prod-ucts. After 3D printing, it is almost always advisable to apply a heat treatment to release the internal tensions or optimize the mechanical properties of the printed parts. The aim of this paper is to investigate the effects of heat treatment of SLM printed Ti-6Al-4V (Ti64) circular specimens on the areal surface texture parameters and cell attachment. Areal surface texture parameters, including the arithmetic mean height (Sa), root-mean-square height (Sq), skewness (Ssk), and kurtosis (Sku) were characterized. In addition, wavelet-based multi-resolution analysis was applied to investigate the characteristic length scales of untreated and heat-treated Ti64 specimens. In this study, the vertical distance between the highest and lowest position of cell attachment for each sampling area was defined as ΔH. Results showed that an increase in the periodic characteristic length scale was pri-marily due to the formation of large-scale aggregations of Ti64 metal powder particles on the heat-treated surface. In addition, MG-63 cells preferred lying in concave hollows; in heat-treated speci-mens, values of ΔH statistically significantly decreased from 31.6 ± 4.2 to 8.8 ± 2.8 μm, while Sku decreased from 3.3 ± 1.4 to 2.6 ± 0.6, indicating a strong influence of Sku on cell attachment.

Original languageEnglish
Article number2234
Pages (from-to)1-14
Number of pages14
JournalApplied Sciences (Switzerland)
Issue number5
Publication statusPublished - Mar 1 2021


  • Cell attachment
  • Heat treatment
  • Microstructure
  • Selective laser melting
  • Surface texture
  • Ti-6Al-4V alloy

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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