TY - JOUR
T1 - Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes
AU - Lin, Yu Hung
AU - Hwang, Jiann-Loung
AU - Seow, Kok Min
AU - Huang, Lee Wen
AU - Chen, Heng Ju
AU - Tzeng, Chii Ruey
N1 - Funding Information:
This study was supported by the Shin Kong Wu Ho-Su Memorial Hospital (SKH-8302–95-DR-20).
PY - 2009/8
Y1 - 2009/8
N2 - The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation with serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell co-culture could overcome the adverse effects of pregnant women's serum. The basal maturation medium consisted of TCM199, 75 mIU/ml human menopausal gonadotrophin (HMG), 0.2 mmol/l pyruvate, and 10% serum. The maturation medium for control 1 contained fertile women's serum. The maturation medium for control 2 contained pregnant women's serum. The maturation media for the study groups consisted of medium for control 2, with the addition of EGF, IGF-I, activin, TGFβ or granulosa cell co-culture. Immature oocytes were obtained from FVB mice, and the experiment was repeated six times. After maturation, the oocytes were fertilized and cultured to blastocysts, and the cumulus cells were analysed for apoptosis. The maturation, fertilization and blastocyst rates of the control 2 group were significantly lower than those of control 1 group (P <0.05). Addition of EGF, IGF-1, activin, TGFβ or granulosa cell co-culture could not improve the outcome of in-vitro maturation. Cumulus cell proliferation was inhibited by pregnant women's serum. Apoptosis of cumulus cell was not related to in-vitro oocyte maturation and subsequent embryo development.
AB - The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation with serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell co-culture could overcome the adverse effects of pregnant women's serum. The basal maturation medium consisted of TCM199, 75 mIU/ml human menopausal gonadotrophin (HMG), 0.2 mmol/l pyruvate, and 10% serum. The maturation medium for control 1 contained fertile women's serum. The maturation medium for control 2 contained pregnant women's serum. The maturation media for the study groups consisted of medium for control 2, with the addition of EGF, IGF-I, activin, TGFβ or granulosa cell co-culture. Immature oocytes were obtained from FVB mice, and the experiment was repeated six times. After maturation, the oocytes were fertilized and cultured to blastocysts, and the cumulus cells were analysed for apoptosis. The maturation, fertilization and blastocyst rates of the control 2 group were significantly lower than those of control 1 group (P <0.05). Addition of EGF, IGF-1, activin, TGFβ or granulosa cell co-culture could not improve the outcome of in-vitro maturation. Cumulus cell proliferation was inhibited by pregnant women's serum. Apoptosis of cumulus cell was not related to in-vitro oocyte maturation and subsequent embryo development.
KW - Apoptosis
KW - Co-culture
KW - Granulosa cell
KW - Growth factor
KW - In-vitro maturation
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U2 - 10.1016/S1472-6483(10)60068-5
DO - 10.1016/S1472-6483(10)60068-5
M3 - Article
C2 - 19712550
AN - SCOPUS:69249138780
SN - 1472-6483
VL - 19
SP - 165
EP - 170
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
IS - 2
ER -