Effects of dietary fatty acid composition on lipid metabolism and body fat accumulation in ovariectomized rats

Jhih Han Yeh, Yu Tang Tung, Yu Sheng Yeh, Yi Wen Chien

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4 Citations (Scopus)


Background: Obesity is a state of excess energy storage resulting in body fat accumulation, and postmenopausal obesity is a rising issue. In this study using ovariectomized (OVX) rats, we mimicked low estrogen levels in a postmenopausal state in order to investigate the effects of different amounts and types of dietary fatty acids on body fat accumulation and body lipid metabolism. Methods: At 9 weeks of age, rats (n = 40) were given an ovariectomy, eight of which were sham-operated to serve as a control group (S). We then divided OVX rats into four different intervention groups: diet with 5% soybean oil (C), and diet with 5% (L), 15% (M), and 20% (H) (w/w) experimental oil, containing 60% monounsaturated fatty acids (MUFAs) and with a polyunsaturated/saturated fatty acid (P/S) ratio of 5. Results: After OVX, compared to the S group, the C group showed significantly higher body weight, and insulin and leptin levels. Compared to the C group, the H group had lower hepatic triglyceride level and FAS enzyme activity, and higher hepatic ACO and CPT‐1 gene expressions and enzyme activities. Conclusions: An OVX leads to severe weight gain and lipid metabolism abnormalities, while according to previous studies, high fat diet may worsen the situa-tion. However, during our experiment, we discovered that the experimental oil mixture with 60% MUFAs and P/S = 5 may ameliorate these imbalances.

Original languageEnglish
Article number2022
Issue number6
Publication statusPublished - Jun 2021


  • Body fat accumulation
  • Lipid metabolism
  • Monounsaturated fatty acids
  • Ovariectomy

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


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