Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice

Sen Wei Tsai, Yi Ju Hsu, Mon Chien Lee, Hao En Huang, Chi Chang Huang, Yu Tang Tung

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Current treatment options for muscle injuries remain suboptimal and often result in delayed/incomplete recovery of damaged muscles. In this study, the effects of dextrose prolotherapy on inflammation and regeneration of skeletal muscles after a contusion injury were investigated. Mice were separated into five groups, including a normal control (NC), post-injury with no treatment (mass-drop injury, MDI), post-injury with 10% dextrose (MDI + 10% dextrose), post-injury with 20% dextrose (MDI + 20% dextrose), and post-injury with 30% dextrose (MDI + 30% dextrose). The gastrocnemius muscles of the mice were subjected to an MDI, and muscle samples were collected at 7 days post-injury. Results showed the serum creatine kinase (CK), blood urea nitrogen (BUN), creatinine (CREA), and low-density lipoprotein (LDH) of the MDI-alone group were significantly higher than those of the normal control group (p<0.05). However, levels of serum CK, BUN, CREA, and lactate dehydrogenase (LDH) significantly decreased with different concentrations of dextrose. In addition, dextrose suppressed the macrophage response (F4/80 protein decreased) and promoted muscle satellite cell regeneration (desmin protein increased). In conclusion, dextrose prolotherapy can effectively help repair muscles; therefore, it may be one of the methods for clinically treating muscle injuries.

Original languageEnglish
Pages (from-to)1251-1259
Number of pages9
JournalInternational Journal of Medical Sciences
Issue number11
Publication statusPublished - Jul 30 2018


  • Contusion
  • Dextrose
  • Diclofenac (DCF)
  • Mass-drop injury (MDI)
  • Prolotherapy
  • Muscle, Skeletal/injuries
  • Male
  • Mice, Inbred ICR
  • Animals
  • Contusions/complications
  • Taiwan
  • Mice
  • Glucose/therapeutic use

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice'. Together they form a unique fingerprint.

Cite this