TY - JOUR
T1 - Effect of soy saponin on the growth of human colon cancer cells
AU - Tsai, Cheng Yu
AU - Chen, Yue Hwa
AU - Chien, Yi Wen
AU - Huang, Wen Hsuan
AU - Lin, Shyh Hsiang
PY - 2010/7/21
Y1 - 2010/7/21
N2 - AIM: To investigate the effect of extracted soybean saponins on the growth of human colon cancer cells. METHODS: WiDr human colon cancer cells were treated with 150, 300, 600 or 1200 ppm of soy saponin to determine the effect on cell growth, cell morphology, alkaline phosphatase (AP) and protein kinase C (PKC) activities, and P53 protein, c-Fos and c-Jun gene expression. RESULTS: Soy saponin decreased the number of viable cells in a dose-dependent manner and suppressed 12-Otetradecanol- phorbol-13-acetate-stimulated PKC activity (P < 0.05). Cells treated with saponins developed cytoplasmic vesicles and the cell membrane became rougher and more irregular in a dose-dependent manner, and eventually disassembled. At 600 and 1200 ppm, the activity of AP was increased (P < 0.05). However, the apoptosis markers such as c-Jun and c-Fos were not significantly affected by saponin. CONCLUSION: Soy saponin may be effective in preventing colon cancer by affecting cell morphology, cell proliferation enzymes, and cell growth.
AB - AIM: To investigate the effect of extracted soybean saponins on the growth of human colon cancer cells. METHODS: WiDr human colon cancer cells were treated with 150, 300, 600 or 1200 ppm of soy saponin to determine the effect on cell growth, cell morphology, alkaline phosphatase (AP) and protein kinase C (PKC) activities, and P53 protein, c-Fos and c-Jun gene expression. RESULTS: Soy saponin decreased the number of viable cells in a dose-dependent manner and suppressed 12-Otetradecanol- phorbol-13-acetate-stimulated PKC activity (P < 0.05). Cells treated with saponins developed cytoplasmic vesicles and the cell membrane became rougher and more irregular in a dose-dependent manner, and eventually disassembled. At 600 and 1200 ppm, the activity of AP was increased (P < 0.05). However, the apoptosis markers such as c-Jun and c-Fos were not significantly affected by saponin. CONCLUSION: Soy saponin may be effective in preventing colon cancer by affecting cell morphology, cell proliferation enzymes, and cell growth.
KW - Apoptosis
KW - Cell differentiation
KW - Cell proliferation
KW - Colon cancer
KW - Soy saponin
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U2 - 10.3748/wjg.v16.i27.3371
DO - 10.3748/wjg.v16.i27.3371
M3 - Article
C2 - 20632438
AN - SCOPUS:77954845339
SN - 1007-9327
VL - 16
SP - 3371
EP - 3376
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 27
ER -
