Effect of reactive oxygen on hyaluronan mediated motility receptor expression in osteoblasts

Aging is a complex biological process driven by a selective class of molecules and pathways that affect the overall deterioration of physiological functions to increase the risk of aging. Bone formation steadily declines with age, resulting in a significant loss of bone mass, and reactive oxygen species (ROS) are thought to be a major contributor to the aging process. In our previous study, H₂O₂ treatment significantly reduced the bone nodule formation rate in the preosteoblast cell line MC3T3-E1. In this study, the effect of H₂O₂ treatment as cellular ageing of osteoblasts on gene expression in MC3T3-E1 cells was examined using DNA microarray technology. Many genes were altered those gene expressions by H₂O₂ treatment. Among these genes, the reduction of the mRNA signal of hyaluronan mediated motility receptor (MHHR) was observed in DNA microarray analysis results. Significantly lower mRNA levels in the gene was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since MHHR involved in bone formation through the stimulation of proliferation and differentiation of osteoblasts, the reduction of gene expression of MHHR by H₂O₂ may be involved in the molecular mechanism of the decline in bone formation with aging.