TY - JOUR
T1 - Effect of laser-assisted reconstructive surgical therapy of peri-implantitis on protein biomarkers and bacterial load
AU - Di Gianfilippo, Riccardo
AU - Wang, Chin Wei
AU - Xie, Yuying
AU - Kinney, Janet
AU - Sugai, James
AU - Giannobile, William V.
AU - Wang, Hom Lay
N1 - Funding Information:
The study was supported by the Department of Periodontics and Oral Medicine (POM) Clinical Research Fund and J. MORITA MFG. CORP., research grant through the University. The authors thank Dr. Sajjad Ashnagar and Ms. Michelle Arnett for their support during the clinical phases of the study. Dr. H‐L Wang is a speaker on behalf of J. MORITA MFG. CORP. and has received honoraria. The other authors do not have any financial interests, either directly or indirectly, in the products or information listed in the article.
Publisher Copyright:
© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2023/4
Y1 - 2023/4
N2 - Objectives: This randomized clinical trial assessed changes in protein biomarker levels and bacterial profiles after surgical reconstructive therapy of peri-implantitis and investigated whether the adjunctive use of Er:YAG laser impacts protein biomarker and microbial outcomes. Materials and Methods: Twenty-four patients received surgical reconstructive therapy for peri-implantitis with guided bone regeneration following mechanical debridement with (test) or without (control) the adjunctive irradiation of Er:YAG laser. Bacterial and peri-implant crevicular fluid (PICF) samples were collected over 6 months and analyzed with bacterial qPCR and luminex multiplex assays. Results: Surgical reconstructive treatment significantly affected the concentration of PICF protein biomarkers, including a 50% reduction in IL-1β between 2 and 4 weeks (p <.0001). Both MMP-9 (p <.001) and VEGF (p <.05) levels steadily decreased after treatment. In the laser group, the peak increase in IL-1β was attenuated at 2 weeks, followed by significant reduction in MMP-9 (p <.01) and VEGF (p <.05) across all follow-up appointments compared with the control nonlaser group. The total bacterial load was reduced 2 weeks after treatment, especially in the laser group, but recolonized to presurgical levels after 4 weeks in both groups (p <.01). The composition of selective pathogens varied significantly over the follow-up, but recolonization patterns did not differ between groups. Conclusions: Reconstructive therapy of peri-implantitis significantly altered PICF protein biomarker and microbial levels during the healing process. The adjunctive use of Er:YAG laser significantly modulated the inflammatory response through reduced levels of MMP-9 and VEGF during the postsurgical period. The bacterial load was reduced immediately after therapy, but recolonization was observed by 4 weeks in both groups.
AB - Objectives: This randomized clinical trial assessed changes in protein biomarker levels and bacterial profiles after surgical reconstructive therapy of peri-implantitis and investigated whether the adjunctive use of Er:YAG laser impacts protein biomarker and microbial outcomes. Materials and Methods: Twenty-four patients received surgical reconstructive therapy for peri-implantitis with guided bone regeneration following mechanical debridement with (test) or without (control) the adjunctive irradiation of Er:YAG laser. Bacterial and peri-implant crevicular fluid (PICF) samples were collected over 6 months and analyzed with bacterial qPCR and luminex multiplex assays. Results: Surgical reconstructive treatment significantly affected the concentration of PICF protein biomarkers, including a 50% reduction in IL-1β between 2 and 4 weeks (p <.0001). Both MMP-9 (p <.001) and VEGF (p <.05) levels steadily decreased after treatment. In the laser group, the peak increase in IL-1β was attenuated at 2 weeks, followed by significant reduction in MMP-9 (p <.01) and VEGF (p <.05) across all follow-up appointments compared with the control nonlaser group. The total bacterial load was reduced 2 weeks after treatment, especially in the laser group, but recolonized to presurgical levels after 4 weeks in both groups (p <.01). The composition of selective pathogens varied significantly over the follow-up, but recolonization patterns did not differ between groups. Conclusions: Reconstructive therapy of peri-implantitis significantly altered PICF protein biomarker and microbial levels during the healing process. The adjunctive use of Er:YAG laser significantly modulated the inflammatory response through reduced levels of MMP-9 and VEGF during the postsurgical period. The bacterial load was reduced immediately after therapy, but recolonization was observed by 4 weeks in both groups.
KW - bone implant interactions
KW - bone regeneration, clinical trials
KW - guided tissue regeneration, bone regeneration
KW - lasers
KW - microbiology
KW - periodontology
KW - wound healing
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U2 - 10.1111/clr.14059
DO - 10.1111/clr.14059
M3 - Article
C2 - 36856540
AN - SCOPUS:85150837699
SN - 0905-7161
VL - 34
SP - 393
EP - 403
JO - Clinical Oral Implants Research
JF - Clinical Oral Implants Research
IS - 4
ER -