TY - JOUR
T1 - Effect of glutathione on DNA repair in cisplatin-resistant human ovarian cancer cell lines
AU - Lai, Gi Ming
AU - Ozols, Robert F.
AU - Young, Robert C.
AU - Hamilton, Thomas C.
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1989/4/5
Y1 - 1989/4/5
N2 - We have studied the effect of glutathione reduction by buthionine sulf-oximine (BSO), a specific inhibitor ofλ -glutamyl cysteine synthetase, on DNA repair after cisplatin damage in an ovarian cancer cell line with invitro induced resistance to cisplatin. In addition, we have examined the effect of aphidicolin, a specific inhibitor of DNA polymerase a, in combination with BSO on cisplatin-associated DNA repair. BSO treatment was found topartially inhibit DNA repair, and the addition of aphidicolin caused nearly a100% inhibition in DNA repair activity. Treatment of cells with glutathioneester after BSO resulted in complete recovery of DNA repair activity or par-tial recovery if aphidicolin was present. The significance of these results to the chemosensitizing effects of BSO medi-ated glutathione reduction is discussed. [J Natl Cancer Inst 81:535-539, 1989].
AB - We have studied the effect of glutathione reduction by buthionine sulf-oximine (BSO), a specific inhibitor ofλ -glutamyl cysteine synthetase, on DNA repair after cisplatin damage in an ovarian cancer cell line with invitro induced resistance to cisplatin. In addition, we have examined the effect of aphidicolin, a specific inhibitor of DNA polymerase a, in combination with BSO on cisplatin-associated DNA repair. BSO treatment was found topartially inhibit DNA repair, and the addition of aphidicolin caused nearly a100% inhibition in DNA repair activity. Treatment of cells with glutathioneester after BSO resulted in complete recovery of DNA repair activity or par-tial recovery if aphidicolin was present. The significance of these results to the chemosensitizing effects of BSO medi-ated glutathione reduction is discussed. [J Natl Cancer Inst 81:535-539, 1989].
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U2 - 10.1093/jnci/81.7.535
DO - 10.1093/jnci/81.7.535
M3 - Article
C2 - 2493524
AN - SCOPUS:0024522119
SN - 0027-8874
VL - 81
SP - 535
EP - 539
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 7
ER -
