Effect of glutaraldehyde on the humoral immunogenicity and structure of porcine dermal collagen membranes

King Jean Wu; Cheng Yi Wang; Hsein Kun Lu

doi:10.1016/j.archoralbio.2003.10.002

Effect of glutaraldehyde on the humoral immunogenicity and structure of porcine dermal collagen membranes

King Jean Wu, Cheng Yi Wang, Hsein Kun Lu

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Objectives: Our previous studies showed that the biodegradation rate of cross-linked porcine dermal collagen membrane (PDCM) could be retarded without changing its biocompatible character. The purpose of this study was to assay the humoral immune response of PDCM reconstitute with glutaraldehyde (GA) and observe their surface architectures. Methods: PDCM reconstituted with GA (0, 0.01, 0.05, and 3%) was implanted in 30 Sprague-Dawley rats. Sample sera were collected 3, 6, and 9 weeks after surgery and assayed with ELISA. The architectures of PDCMs were observed under SEM (100×). Results: The study showed that non cross-linked PDCM induced the highest immune response than any other cross-linked PDCMs (by optical density (OD) values, P

Original language	English
Pages (from-to)	305-311
Number of pages	7
Journal	Archives of Oral Biology
Volume	49
Issue number	4
DOIs	https://doi.org/10.1016/j.archoralbio.2003.10.002
Publication status	Published - Apr 2004

Keywords

ELISA
Glutaraldehyde crosslinking
Immunogenicity
PDCM

ASJC Scopus subject areas

General Dentistry
Cell Biology
Otorhinolaryngology

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10.1016/j.archoralbio.2003.10.002

Cite this

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title = "Effect of glutaraldehyde on the humoral immunogenicity and structure of porcine dermal collagen membranes",

abstract = "Objectives: Our previous studies showed that the biodegradation rate of cross-linked porcine dermal collagen membrane (PDCM) could be retarded without changing its biocompatible character. The purpose of this study was to assay the humoral immune response of PDCM reconstitute with glutaraldehyde (GA) and observe their surface architectures. Methods: PDCM reconstituted with GA (0, 0.01, 0.05, and 3%) was implanted in 30 Sprague-Dawley rats. Sample sera were collected 3, 6, and 9 weeks after surgery and assayed with ELISA. The architectures of PDCMs were observed under SEM (100×). Results: The study showed that non cross-linked PDCM induced the highest immune response than any other cross-linked PDCMs (by optical density (OD) values, P",

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T1 - Effect of glutaraldehyde on the humoral immunogenicity and structure of porcine dermal collagen membranes

AU - Wu, King Jean

AU - Wang, Cheng Yi

AU - Lu, Hsein Kun

PY - 2004/4

Y1 - 2004/4

N2 - Objectives: Our previous studies showed that the biodegradation rate of cross-linked porcine dermal collagen membrane (PDCM) could be retarded without changing its biocompatible character. The purpose of this study was to assay the humoral immune response of PDCM reconstitute with glutaraldehyde (GA) and observe their surface architectures. Methods: PDCM reconstituted with GA (0, 0.01, 0.05, and 3%) was implanted in 30 Sprague-Dawley rats. Sample sera were collected 3, 6, and 9 weeks after surgery and assayed with ELISA. The architectures of PDCMs were observed under SEM (100×). Results: The study showed that non cross-linked PDCM induced the highest immune response than any other cross-linked PDCMs (by optical density (OD) values, P

AB - Objectives: Our previous studies showed that the biodegradation rate of cross-linked porcine dermal collagen membrane (PDCM) could be retarded without changing its biocompatible character. The purpose of this study was to assay the humoral immune response of PDCM reconstitute with glutaraldehyde (GA) and observe their surface architectures. Methods: PDCM reconstituted with GA (0, 0.01, 0.05, and 3%) was implanted in 30 Sprague-Dawley rats. Sample sera were collected 3, 6, and 9 weeks after surgery and assayed with ELISA. The architectures of PDCMs were observed under SEM (100×). Results: The study showed that non cross-linked PDCM induced the highest immune response than any other cross-linked PDCMs (by optical density (OD) values, P

KW - ELISA

KW - Glutaraldehyde crosslinking

KW - Immunogenicity

KW - PDCM

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Effect of glutaraldehyde on the humoral immunogenicity and structure of porcine dermal collagen membranes

Abstract

Keywords

ASJC Scopus subject areas

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