TY - JOUR
T1 - Effect of ethanol on the electrophysiological characteristics of pulmonary vein cardiomyocytes
AU - Chen, Yao Chang
AU - Chen, Shih Ann
AU - Chen, Yi Jen
AU - Tai, Ching Tai
AU - Chan, Paul
AU - Lin, Cheng I.
PY - 2004/1/12
Y1 - 2004/1/12
N2 - Ethanol consumption has been considered to contribute to the occurrences of paroxysmal atrial fibrillation. Pulmonary veins are known to initiate atrial fibrillation. This study investigated whether ethanol may induce atrial fibrillation through increasing arrhythmogenic activity of pulmonary vein cardiomyocytes. Using the whole-cell clamp technique, the action potential and ionic currents were investigated in rabbit single pulmonary vein beating cardiomyocytes with and without (control) incubation of ethanol. Compared with control cardiomyocytes, pulmonary vein cardiomyocytes receiving 0.3 mg/ml or 1 mg/ml ethanol had shorter action potential duration, but had similar beating rates (2.6±1.3, 2.7±1.2, 2.7±1.2 Hz) and incidences (45%, 41%, 32%) of delayed afterdepolarization. Pulmonary vein cardiomyocytes receiving ethanol had smaller L-type Ca2+ currents and larger transient outward currents, but had similar transient inward, delayed rectified outward, inward rectified and pacemaker currents. These results suggest that ethanol has no direct effect on the arrhythmogenic potential of pulmonary vein cardiomyocytes.
AB - Ethanol consumption has been considered to contribute to the occurrences of paroxysmal atrial fibrillation. Pulmonary veins are known to initiate atrial fibrillation. This study investigated whether ethanol may induce atrial fibrillation through increasing arrhythmogenic activity of pulmonary vein cardiomyocytes. Using the whole-cell clamp technique, the action potential and ionic currents were investigated in rabbit single pulmonary vein beating cardiomyocytes with and without (control) incubation of ethanol. Compared with control cardiomyocytes, pulmonary vein cardiomyocytes receiving 0.3 mg/ml or 1 mg/ml ethanol had shorter action potential duration, but had similar beating rates (2.6±1.3, 2.7±1.2, 2.7±1.2 Hz) and incidences (45%, 41%, 32%) of delayed afterdepolarization. Pulmonary vein cardiomyocytes receiving ethanol had smaller L-type Ca2+ currents and larger transient outward currents, but had similar transient inward, delayed rectified outward, inward rectified and pacemaker currents. These results suggest that ethanol has no direct effect on the arrhythmogenic potential of pulmonary vein cardiomyocytes.
KW - Action potential
KW - Atrial fibrillation
KW - Ethanol
KW - Pulmonary vein
UR - http://www.scopus.com/inward/record.url?scp=0347318071&partnerID=8YFLogxK
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U2 - 10.1016/j.ejphar.2003.10.042
DO - 10.1016/j.ejphar.2003.10.042
M3 - Article
C2 - 14729109
AN - SCOPUS:0347318071
SN - 0014-2999
VL - 483
SP - 215
EP - 222
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 2-3
ER -