Effect of different resuscitation fluids on cytokine response in a rat model of hemorrhagic shock

Chien Chang Lee, I. Jing Chang, Zui Shen Yen, Chiung Yuan Hsu, Shey Yiny Chen, Chan Ping Su, Wen Chu Chiang, Shyr Chyr Chen, Wen Jone Chen

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45 Citations (Scopus)


This study was designed to determine the effects of different resuscitation fluids on the production of proinflammatory and anti-inflammatory cytokines in an animal model of hemorrhagic shock. Wistar male rats (n = 24; 8/group) were subjected to a volume-controlled hemorrhagic shock for 30 minutes and resuscitated as follows: (1) sham group without resuscitation, (2) lactated Ringer solution (LR), 3:1; (3) 4% hydroxyethyl starch (HES) solution, 1:1; and (4) 4% modified fluid gelatin (GEL), 1:1. Hemodynamic parameters were recorded, and blood samples were collected at 0 min and 30, 90, 150, 210, 270, and 330 min after hemorrhage for plasma levels of IL-6, IL-10, and TNFα. The circulating concentrations of IL-6 at 90, 150, 210, 270, and 330 min and TNFα levels at 150, 210, and 270 min after hemorrhage were significantly elevated in animals resuscitated with GEL compared with HES or LR (P < 0.05). At 210, 270, and 330 min, IL-10 concentration was decreased significantly in GEL-resuscitated rats compared with rats resuscitated with LR or HES (P < 0.05). Mean blood pressure and serum levels of lactate after resuscitation were not different among three kinds of fluids. LR, HES, and GEL are comparable in volume efficacy for resuscitation of hemorrhagic shock but are associated with different postresuscitation immune responses. Resuscitation with GEL may be associated with cytokine production favoring a proinflammatory response. The marked elevation of IL-6 observed in the GEL-treated animals may play a role in the relatively high frequency of anaphylactoid reaction in clinical use of GEL.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
Issue number2
Publication statusPublished - Aug 1 2005
Externally publishedYes


  • Anaphylactoid reaction
  • Cytokine
  • Gelatin
  • Hemorrhagic shock
  • Hydroxyethyl starch
  • Lactated Ringer
  • Resuscitation fluids

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine


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