Dynamic change of surface microbiota with different environmental cleaning methods between two wards in a hospital

Chang Hua Chen, Chi Chao Tu, Han Yueh Kuo, Rong Fong Zeng, Cheng Sheng Yu, Henry Horng Shing Lu, Ming Li Liou

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes of bacterial communities with terminal disinfection and daily cleaning in a medical intensive care unit (MICU) and only terminal disinfection in a respiratory care center (RCC) using 16s ribosomal RNA (rRNA) metagenomics. A total of 36 samples, including 9 samples per sampling time, from each ward were analysed. The clinical isolates were recorded during the sampling time. A large amount of microbial diversity was detected, and human skin microbiota (HSM) was predominant in both wards. In addition, the colonization rate of the HSM in the MICU was higher than that in the RCC, especially for Moraxellaceae. A higher alpha-diversity (p = 0.005519) and a lower UniFrac distance was shown in the RCC due to the lack of daily cleaning. Moreover, a significantly higher abundance among Acinetobacter sp., Streptococcus sp. and Pseudomonas sp. was shown in the RCC compared to the MICU using the paired t test. We concluded that cleaning changes might contribute to the difference in diversity between two wards.

Original languageEnglish
Pages (from-to)771-781
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume101
Issue number2
DOIs
Publication statusPublished - Jan 2017
Externally publishedYes

Keywords

  • 16S rRNA metagenomics
  • Acinetobacter
  • Environmental cleaning methods
  • Healthcare-associated infection
  • Medical intensive care unit
  • Respiratory care centre

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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