Dose-dependent effects of adipose tissue-derived stromal vascular fraction cells on angiogenesis and fibrosis in human fat grafts

Shou Cheng Teng; Li Ting Li; Shyi Gen Chen; Tien Mu Chen; Cheng Hao Liao; Hsu Wei Fang

doi:10.4015/S1016237214500458

Dose-dependent effects of adipose tissue-derived stromal vascular fraction cells on angiogenesis and fibrosis in human fat grafts

Shou Cheng Teng, Li Ting Li, Shyi Gen Chen, Tien Mu Chen, Cheng Hao Liao, Hsu Wei Fang

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Adipose tissue is not only an ideal material for soft tissue filling and augmentation, but also a plentiful source of regenerative cells in adipose-derived stromal vascular fraction. Enriched fat tissue with adipose-derived stromal vascular fraction cells (ADSVFCs) can improve the fat graft survival, as shown in previous studies. The objective of this study is to estimate the effects of various dosages of ADSVFCs on fat grafts. We hypothesized that the improvement of ADSVFCs on fat transplantation quality would be dose dependent. Fat tissues were obtained by liposuction surgery. ADSVFCs were mixed into fat tissue, and the ADSVFC-enriched fat tissues were implanted subcutaneously into nude mice. The histological findings showed that the neoangiogenesis and integrity of grafted fat cells significantly increased with increasing dosages of ADSVFCs. Higher dosages of ADSVFCs also significantly reduced the side effects of the fat grafts, including reducing inflammation, cell infiltration, fibrosis and cyst formation. In conclusion, ADSVFC supplemented fat implants can improve angiogenesis and anti-inflammation. However, these effects of ADSVFCs on fat graft quality are dose dependent.

Original language	English
Article number	1450045
Journal	Biomedical Engineering - Applications, Basis and Communications
Volume	26
Issue number	3
DOIs	https://doi.org/10.4015/S1016237214500458
Publication status	Published - Jan 1 2014
Externally published	Yes

Keywords

Adipose tissue transplantation
Adipose-derived stromal vascular fraction cells
Fat graft
Lipotransfer

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomedical Engineering

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title = "Dose-dependent effects of adipose tissue-derived stromal vascular fraction cells on angiogenesis and fibrosis in human fat grafts",

abstract = "Adipose tissue is not only an ideal material for soft tissue filling and augmentation, but also a plentiful source of regenerative cells in adipose-derived stromal vascular fraction. Enriched fat tissue with adipose-derived stromal vascular fraction cells (ADSVFCs) can improve the fat graft survival, as shown in previous studies. The objective of this study is to estimate the effects of various dosages of ADSVFCs on fat grafts. We hypothesized that the improvement of ADSVFCs on fat transplantation quality would be dose dependent. Fat tissues were obtained by liposuction surgery. ADSVFCs were mixed into fat tissue, and the ADSVFC-enriched fat tissues were implanted subcutaneously into nude mice. The histological findings showed that the neoangiogenesis and integrity of grafted fat cells significantly increased with increasing dosages of ADSVFCs. Higher dosages of ADSVFCs also significantly reduced the side effects of the fat grafts, including reducing inflammation, cell infiltration, fibrosis and cyst formation. In conclusion, ADSVFC supplemented fat implants can improve angiogenesis and anti-inflammation. However, these effects of ADSVFCs on fat graft quality are dose dependent.",

keywords = "Adipose tissue transplantation, Adipose-derived stromal vascular fraction cells, Fat graft, Lipotransfer",

author = "Teng, {Shou Cheng} and Li, {Li Ting} and Chen, {Shyi Gen} and Chen, {Tien Mu} and Liao, {Cheng Hao} and Fang, {Hsu Wei}",

note = "Funding Information: This study was supported by a grant TSGH-C100-013-S03 from the Tri-Service General Hospital, Taiwan. Shou-Cheng Teng and Li-Ting Li contributed equally to this work.",

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T1 - Dose-dependent effects of adipose tissue-derived stromal vascular fraction cells on angiogenesis and fibrosis in human fat grafts

AU - Teng, Shou Cheng

AU - Li, Li Ting

AU - Chen, Shyi Gen

AU - Chen, Tien Mu

AU - Liao, Cheng Hao

AU - Fang, Hsu Wei

N1 - Funding Information: This study was supported by a grant TSGH-C100-013-S03 from the Tri-Service General Hospital, Taiwan. Shou-Cheng Teng and Li-Ting Li contributed equally to this work.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Adipose tissue is not only an ideal material for soft tissue filling and augmentation, but also a plentiful source of regenerative cells in adipose-derived stromal vascular fraction. Enriched fat tissue with adipose-derived stromal vascular fraction cells (ADSVFCs) can improve the fat graft survival, as shown in previous studies. The objective of this study is to estimate the effects of various dosages of ADSVFCs on fat grafts. We hypothesized that the improvement of ADSVFCs on fat transplantation quality would be dose dependent. Fat tissues were obtained by liposuction surgery. ADSVFCs were mixed into fat tissue, and the ADSVFC-enriched fat tissues were implanted subcutaneously into nude mice. The histological findings showed that the neoangiogenesis and integrity of grafted fat cells significantly increased with increasing dosages of ADSVFCs. Higher dosages of ADSVFCs also significantly reduced the side effects of the fat grafts, including reducing inflammation, cell infiltration, fibrosis and cyst formation. In conclusion, ADSVFC supplemented fat implants can improve angiogenesis and anti-inflammation. However, these effects of ADSVFCs on fat graft quality are dose dependent.

AB - Adipose tissue is not only an ideal material for soft tissue filling and augmentation, but also a plentiful source of regenerative cells in adipose-derived stromal vascular fraction. Enriched fat tissue with adipose-derived stromal vascular fraction cells (ADSVFCs) can improve the fat graft survival, as shown in previous studies. The objective of this study is to estimate the effects of various dosages of ADSVFCs on fat grafts. We hypothesized that the improvement of ADSVFCs on fat transplantation quality would be dose dependent. Fat tissues were obtained by liposuction surgery. ADSVFCs were mixed into fat tissue, and the ADSVFC-enriched fat tissues were implanted subcutaneously into nude mice. The histological findings showed that the neoangiogenesis and integrity of grafted fat cells significantly increased with increasing dosages of ADSVFCs. Higher dosages of ADSVFCs also significantly reduced the side effects of the fat grafts, including reducing inflammation, cell infiltration, fibrosis and cyst formation. In conclusion, ADSVFC supplemented fat implants can improve angiogenesis and anti-inflammation. However, these effects of ADSVFCs on fat graft quality are dose dependent.

KW - Adipose tissue transplantation

KW - Adipose-derived stromal vascular fraction cells

KW - Fat graft

KW - Lipotransfer

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Dose-dependent effects of adipose tissue-derived stromal vascular fraction cells on angiogenesis and fibrosis in human fat grafts

Abstract

Keywords

ASJC Scopus subject areas

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