Dobutamine inhibits monocyte chemoattractant protein-1 production and chemotaxis in human monocytes

Chi Yuan Li, Chien Sung Tsai, Sheau Huei Chueh, Ping Ching Hsu, Jia Yi Wang, Chih Shung Wong, Shung Tai Ho

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7 Citations (Scopus)


It has been reported that, in patients with acute myocardial infarction or congestive heart failure, monocyte chemoattractant protein-1 (MCP-1) plays an important role in the development of inflammatory responses and that the level of MCP-1 is correlated with the severity of the disease. We conducted this study to investigate the effects of dobutamine and dopamine on lipopolysaccharide (LPS)-induced MCP-1 production in human monocytic THP-1 cells. Monocytes were incubated in vitro with LPS for 16 h at 37°C in the presence or absence of dobutamine or dopamine. Enzyme-linked immunosorbent assay was used to examine the effect of dobutamine on MCP-1 synthesis, with the MCP-1 messenger RNA expression examined by reverse transcriptase-polymerase chain reaction. Dobutamine inhibited LPS-induced production of MCP-1, as well as messenger RNA expression, in a dose-dependent manner, whereas dopamine had no significant effect. Furthermore, we demonstrated that dobutamine suppressed MCP-1-induced chemotaxis and peak [Ca2+]i in monocytic THP-1 cells. These findings suggest that dobutamine may modulate monocyte activation, such as chemotaxis and [Ca2+]i, as well as MCP-1 production, during therapy for congestive heart failure.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalAnesthesia and Analgesia
Issue number1
Publication statusPublished - Jul 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine


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